Secretion of IL-12 p40 and p70 Into Whole Blood
| . | . | IL-12 p40 (pg/mL) . | IL-12 p70 (pg/mL) . | ||
|---|---|---|---|---|---|
| . | . | LPS . | LPS + rhIFN-γ . | LPS . | LPS + rhIFN-γ . |
| 8 h | Control | 691.1 ± 122.7 | 1,518.8 ± 319.2* | 10.0 ± 4.5 | 236.6 ± 63.8* |
| Patient | 46.6 ± 6.2† | 159.0 ± 31.2*† | ND | ND† | |
| 24 h | Control | 964.9 ± 185.8 | 1,673.7 ± 254.5* | 9.0 ± 3.5 | 299.8 ± 105.1* |
| Patient | 52.7 ± 6.8† | 223.1 ± 47.0*† | ND | ND† | |
| . | . | IL-12 p40 (pg/mL) . | IL-12 p70 (pg/mL) . | ||
|---|---|---|---|---|---|
| . | . | LPS . | LPS + rhIFN-γ . | LPS . | LPS + rhIFN-γ . |
| 8 h | Control | 691.1 ± 122.7 | 1,518.8 ± 319.2* | 10.0 ± 4.5 | 236.6 ± 63.8* |
| Patient | 46.6 ± 6.2† | 159.0 ± 31.2*† | ND | ND† | |
| 24 h | Control | 964.9 ± 185.8 | 1,673.7 ± 254.5* | 9.0 ± 3.5 | 299.8 ± 105.1* |
| Patient | 52.7 ± 6.8† | 223.1 ± 47.0*† | ND | ND† | |
Whole blood obtained from healthy individuals (controls, n = 12) and critically ill patients (n = 25) was incubated with LPS (1 ng/mL) in the presence or absence of rhIFN-γ (10 ng/mL) for 8 hours and 24 hours, respectively. Plasma levels of IL-12 p40 (pg/mL) and p70 (pg/mL) were measured using specific ELISA. Data are presented as mean ± SEM.
Abbreviation: ND, not detectable.
P < .05 LPS versus LPS + rhIFN-γ.
P < .05 patient versus control.