Effect of EBV on 5-Lipoxygenase Activity in Human PBMCs
| Experiment No. . | . | Stimuli . | |
|---|---|---|---|
| . | . | A23187 . | EBV + A23187 . |
| 1 | LTB4 | 3 ± 1 | 27 ± 2 |
| 5,15-DiHETE | 129 ± 46 | 296 ± 17 | |
| 2 | LTB4 | ND | 21 ± 6 |
| 5,15-DiHETE | ND | 120 ± 28 | |
| 3 | LTB4 | ND | 43 ± 4 |
| 5,15-DiHETE | ND | 361 ± 21 | |
| 4 | LTB4 | 17 ± 1 | 71 ± 5 |
| 5,15-DiHETE | 280 ± 21 | 379 ± 44 | |
| Experiment No. . | . | Stimuli . | |
|---|---|---|---|
| . | . | A23187 . | EBV + A23187 . |
| 1 | LTB4 | 3 ± 1 | 27 ± 2 |
| 5,15-DiHETE | 129 ± 46 | 296 ± 17 | |
| 2 | LTB4 | ND | 21 ± 6 |
| 5,15-DiHETE | ND | 120 ± 28 | |
| 3 | LTB4 | ND | 43 ± 4 |
| 5,15-DiHETE | ND | 361 ± 21 | |
| 4 | LTB4 | 17 ± 1 | 71 ± 5 |
| 5,15-DiHETE | 280 ± 21 | 379 ± 44 | |
PBMCs (107/0.1 mL) were preincubated with or without EBV for 20 minutes, diluted to 1 mL with HBSS, and stimulated with A23187 (75 nmol/L) for 5 minutes in the presence of 15-HpETE (3 μmol/L). Reactions were stopped by the addition of 1 vol of methanol/acetonitrile (50/50, vol/vol) and the LTB4 and 5,15-DiHETE contents of incubation media were measured by RP-HPLC. 5-LO products were not detectable in untreated cells or in cells treated with EBV alone. Values are the means of triplicate incubations ± SD and are expressed in picomoles per 107 cells.
Abbreviation: ND, not detectable.