Effect of EBV on LTB4 Synthesis in Human PBMCs
| Experiment No. . | Stimuli . | |||||
|---|---|---|---|---|---|---|
| . | fMLP . | EBV + fMLP . | Zymosan . | EBV + Zymosan . | A23187 . | EBV + A23187 . |
| 1 | ND | 8.2 ± 0.4 | 2.8 ± 1.6 | 15.8 ± 2.4 | ND | 42.0 ± 10.0 |
| 2 | ND | 10.0 ± 1.2 | 6.6 ± 1.2 | 21.4 ± 2.8 | 46.0 ± 9.0 | 320.0 ± 10.0 |
| 3 | 1.2 ± 0.4 | 10.2 ± 2.4 | 2.8 ± 0.2 | 11.2 ± 2.8 | 12.2 ± 2.6 | 169.4 ± 27.4 |
| 4 | 1.6 ± 0.6 | 12.6 ± 0.6 | 5.0 ± 0.6 | 24.6 ± 1.2 | 66.2 ± 6.0 | 151.8 ± 24.4 |
| 5 | ND | 10.6 ± 0.4 | ND | 9.0 ± 1.4 | 9.0 ± 4.0 | 122.4 ± 10.2 |
| Experiment No. . | Stimuli . | |||||
|---|---|---|---|---|---|---|
| . | fMLP . | EBV + fMLP . | Zymosan . | EBV + Zymosan . | A23187 . | EBV + A23187 . |
| 1 | ND | 8.2 ± 0.4 | 2.8 ± 1.6 | 15.8 ± 2.4 | ND | 42.0 ± 10.0 |
| 2 | ND | 10.0 ± 1.2 | 6.6 ± 1.2 | 21.4 ± 2.8 | 46.0 ± 9.0 | 320.0 ± 10.0 |
| 3 | 1.2 ± 0.4 | 10.2 ± 2.4 | 2.8 ± 0.2 | 11.2 ± 2.8 | 12.2 ± 2.6 | 169.4 ± 27.4 |
| 4 | 1.6 ± 0.6 | 12.6 ± 0.6 | 5.0 ± 0.6 | 24.6 ± 1.2 | 66.2 ± 6.0 | 151.8 ± 24.4 |
| 5 | ND | 10.6 ± 0.4 | ND | 9.0 ± 1.4 | 9.0 ± 4.0 | 122.4 ± 10.2 |
PBMCs from healthy donors were incubated with EBV for 20 minutes, diluted to 1 mL with HBSS, and stimulated with zymosan (0.1 mg/mL for 30 minutes), fMLP (1 μmol/L for 10 minutes), or A23187 (75 nmol/L for 5 minutes). Reactions were stopped by the addition of 1 vol of methanol/acetonitrile (50/50, vol/vol) and the LTB4 content of incubation media was measured by RP-HPLC. Values are the means of triplicate incubations ± SD and are expressed in picomoles per 10 × 106 cells. LTB4 synthesis was not detectable in untreated cells or in cells treated with EBV only.
Abbreviation: ND, not detectable.