Reconstituting Ability of Fresh Bone Marrow Cells and Day-14 Cultured Cells
| A. Quantitation of Donor Cells . | ||||
|---|---|---|---|---|
| . | . | . | . | . |
| Cytokines in Culture . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells3-150 . | |
| . | . | . | 2 Months . | 6 Months . |
| Summary Data | ||||
| Fresh marrow | 100 | 41 | 45.1 ± 7.0 | 41.1 ± 9.8 |
| 10 | 11.0 ± 13.6 | 7.1 ± 8.9 | ||
| 1 | 2.5 ± 3.2 | 2.7 ± 4.1 | ||
| FL, KL | 660 | ND | 0.5 ± 0.1 | 0.6 ± 0.1 |
| FL, IL-11 | 5.0 × 105 | 4,630 | 59.0 ± 15.8 | 72.3 ± 23.9 |
| 5.0 × 104 | 11.8 ± 15.7 | 18.5 ± 26.9 | ||
| 5.0 × 103 | 1.1 ± 0.9 | 1.1 ± 0.6 | ||
| KL, IL-11 | 2.0 × 106 | 15,300 | 57.1 ± 19.6 | 50.0 ± 27.2 |
| 2.0 × 105 | 4.6 ± 2.4 | 1.9 ± 0.7 | ||
| 2.0 × 104 | 0.3 ± 0.1 | 0.6 ± 0.1 | ||
| Individual Recipient Data | ||||
| Fresh marrow | 100 | 35.6 | 27.9 | |
| 55.2 | 57.2 | |||
| 41.2 | 40.3 | |||
| 50.7 | 35.1 | |||
| 42.8 | 44.8 | |||
| FL, IL-11 | 5.0 × 105 | 79.3 | 92.8 | |
| 69.3 | 84.0 | |||
| 32.8 | 28.8 | |||
| 60.7 | 90.8 | |||
| 53.0 | 65.0 | |||
| KL, IL-11 | 2.0 × 106 | 82.1 | 80.2 | |
| 42.2 | 15.4 | |||
| 78.1 | 80.1 | |||
| 32.8 | 23.6 | |||
| 50.2 | 50.5 | |||
| B. Surface Phenotype of Donor Cells | ||||
| Cytokines in Culture | % Mac-1+Gr-1+ Cells | % B 220+ Cells | % Thy-1+ Cells | |
| Fresh marrow | 3.1 | 30.5 | 20.8 | |
| 2.5 | 22.3 | 13.2 | ||
| FL, IL-11 | 8.5 | 42.0 | 28.5 | |
| 6.0 | 48.0 | 25.2 | ||
| KL, IL-11 | 2.1 | 32.5 | 26.5 | |
| 4.0 | 25.3 | 21.5 | ||
| A. Quantitation of Donor Cells . | ||||
|---|---|---|---|---|
| . | . | . | . | . |
| Cytokines in Culture . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells3-150 . | |
| . | . | . | 2 Months . | 6 Months . |
| Summary Data | ||||
| Fresh marrow | 100 | 41 | 45.1 ± 7.0 | 41.1 ± 9.8 |
| 10 | 11.0 ± 13.6 | 7.1 ± 8.9 | ||
| 1 | 2.5 ± 3.2 | 2.7 ± 4.1 | ||
| FL, KL | 660 | ND | 0.5 ± 0.1 | 0.6 ± 0.1 |
| FL, IL-11 | 5.0 × 105 | 4,630 | 59.0 ± 15.8 | 72.3 ± 23.9 |
| 5.0 × 104 | 11.8 ± 15.7 | 18.5 ± 26.9 | ||
| 5.0 × 103 | 1.1 ± 0.9 | 1.1 ± 0.6 | ||
| KL, IL-11 | 2.0 × 106 | 15,300 | 57.1 ± 19.6 | 50.0 ± 27.2 |
| 2.0 × 105 | 4.6 ± 2.4 | 1.9 ± 0.7 | ||
| 2.0 × 104 | 0.3 ± 0.1 | 0.6 ± 0.1 | ||
| Individual Recipient Data | ||||
| Fresh marrow | 100 | 35.6 | 27.9 | |
| 55.2 | 57.2 | |||
| 41.2 | 40.3 | |||
| 50.7 | 35.1 | |||
| 42.8 | 44.8 | |||
| FL, IL-11 | 5.0 × 105 | 79.3 | 92.8 | |
| 69.3 | 84.0 | |||
| 32.8 | 28.8 | |||
| 60.7 | 90.8 | |||
| 53.0 | 65.0 | |||
| KL, IL-11 | 2.0 × 106 | 82.1 | 80.2 | |
| 42.2 | 15.4 | |||
| 78.1 | 80.1 | |||
| 32.8 | 23.6 | |||
| 50.2 | 50.5 | |||
| B. Surface Phenotype of Donor Cells | ||||
| Cytokines in Culture | % Mac-1+Gr-1+ Cells | % B 220+ Cells | % Thy-1+ Cells | |
| Fresh marrow | 3.1 | 30.5 | 20.8 | |
| 2.5 | 22.3 | 13.2 | ||
| FL, IL-11 | 8.5 | 42.0 | 28.5 | |
| 6.0 | 48.0 | 25.2 | ||
| KL, IL-11 | 2.1 | 32.5 | 26.5 | |
| 4.0 | 25.3 | 21.5 | ||
Irradiated recipients (5 mice per group) were transplanted with 100, 10, or one cell equivalents of fresh Lin−Sca-1+c-kit+ cells or with whole or fractions of expanded cultures initiated with 100 Lin−Sca-1+c-kit+. Levels of engraftment at 2 and 6 months are shown both as a summary of groups and as individual data. Lineage phenotypes of the donor cells were analyzed at 6 months in 2 mice per group.
Mean ± SD % donor-derived nucleated peripheral blood cells identified by anti-Ly5.1. Background (peripheral blood cells of nontransplanted female mice) staining was <1.0%.