Expansion of Cell and Progenitor Populations in Suspension Culture
| Cytokines . | Day 7 . | Day 14 . | ||||
|---|---|---|---|---|---|---|
| . | TNC . | CFU-C . | CFU-GEMM . | TNC . | CFU-C . | CFU-GEMM . |
| FL | <1,000 | ND | ND | <1,000 | ND | ND |
| KL | 3.4 × 103 | 400 | 50 | 3.2 × 103 | 110 | 10 |
| IL-11 | 0 | 0 | 0 | 0 | 0 | 0 |
| FL,KL | 5.6 × 103 | 580 | 30 | 1.3 × 104 | 110 | 10 |
| FL, IL-11 | 5.6 × 104 | 7,800 | 180 | 2.2 × 107 | 228,000 | 2,500 |
| KL, IL-11 | 2.2 × 105 | 33,700 | 1,050 | 1.1 × 108 | 706,000 | 12,000 |
| Cytokines . | Day 7 . | Day 14 . | ||||
|---|---|---|---|---|---|---|
| . | TNC . | CFU-C . | CFU-GEMM . | TNC . | CFU-C . | CFU-GEMM . |
| FL | <1,000 | ND | ND | <1,000 | ND | ND |
| KL | 3.4 × 103 | 400 | 50 | 3.2 × 103 | 110 | 10 |
| IL-11 | 0 | 0 | 0 | 0 | 0 | 0 |
| FL,KL | 5.6 × 103 | 580 | 30 | 1.3 × 104 | 110 | 10 |
| FL, IL-11 | 5.6 × 104 | 7,800 | 180 | 2.2 × 107 | 228,000 | 2,500 |
| KL, IL-11 | 2.2 × 105 | 33,700 | 1,050 | 1.1 × 108 | 706,000 | 12,000 |
One thousand Lin−Sca-1+c-kit+ cells were cultured in the presence of the designated cytokines. On day 7, the cells were washed and then diluted in freshly prepared media, and the incubation continued for 7 more days. On both day 7 and day 14 of culture, cells were analyzed for CFU-C and CFU-GEMM in methylcellulose culture.
Abbreviation: ND, not determined.