Affinity of IL-3 for the Different Receptors
| Cell Type . | kd of High-Affinity Site (and receptors/cell) . | ||
|---|---|---|---|
| . | IL-3Rα + hβc . | IL-3RαΔNT + hβc . | IL-3RαΔCT + hβc . |
| COS | 113 pmol/L | — | — |
| COS | 35 pmol/L | — | — |
| COS | 110 pmol/L | 1,360 pmol/L | — |
| COS | 130 pmol/L | 1,200 pmol/L | — |
| FDC-P1 | 45 pmol/L (7,770) | — | — |
| FDC-P1 | 59 pmol/L (8,030) | — | — |
| FDC-P1 | — | 868 pmol/L (4,113) | — |
| FDC-P1 | — | 1,129 pmol/L (5,560) | — |
| FDC-P1 | — | 1,600 pmol/L (946) | 224 pmol/L |
| (2,040) | |||
| Mean ± SD | 82 ± 36.9 pmol/L | 1,231 ± 243 pmol/L | |
| Cell Type . | kd of High-Affinity Site (and receptors/cell) . | ||
|---|---|---|---|
| . | IL-3Rα + hβc . | IL-3RαΔNT + hβc . | IL-3RαΔCT + hβc . |
| COS | 113 pmol/L | — | — |
| COS | 35 pmol/L | — | — |
| COS | 110 pmol/L | 1,360 pmol/L | — |
| COS | 130 pmol/L | 1,200 pmol/L | — |
| FDC-P1 | 45 pmol/L (7,770) | — | — |
| FDC-P1 | 59 pmol/L (8,030) | — | — |
| FDC-P1 | — | 868 pmol/L (4,113) | — |
| FDC-P1 | — | 1,129 pmol/L (5,560) | — |
| FDC-P1 | — | 1,600 pmol/L (946) | 224 pmol/L |
| (2,040) | |||
| Mean ± SD | 82 ± 36.9 pmol/L | 1,231 ± 243 pmol/L | |
Each line represents a separate experiment. For COS cells, each Scatchard analysis involved a separate transfection; for FDC-P1 cells the same stably transfected lines were analyzed several times.