Table 1.

rAAV-Mediated Globin Gene Transfer Into Erythroleukemia (K562) Cells

MOImRNA (RT-PCR)Integration (Southern blot)*
Day 18†Day 35†Integrated†Unrearranged‡Tandem Copiesρ
rHS432Aγ* 3 × 108 12/12 19/23 9/16 5/9 0/5 
rHS32Aγ*3′RE 4 × 108 10/10 ND 8/18 8/8 6/8 
rHS32Aγ*3′RE 4 × 109 10/10 ND 23/24 23/23 22/23 
MOImRNA (RT-PCR)Integration (Southern blot)*
Day 18†Day 35†Integrated†Unrearranged‡Tandem Copiesρ
rHS432Aγ* 3 × 108 12/12 19/23 9/16 5/9 0/5 
rHS32Aγ*3′RE 4 × 108 10/10 ND 8/18 8/8 6/8 
rHS32Aγ*3′RE 4 × 109 10/10 ND 23/24 23/23 22/23 

Abbreviation: ND, not determined.

*

Southern blot analysis was performed on DNA extracted 35 to 50 days after transduction.

†

Ratio of positive clones to number of clones analyzed.

‡

Proportion of clones that contain an unrearranged rAAV genome.

ρ Proportion of clones in which the integrated, intact genome was in a tandem array of multiple copies.

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