Flow Cytometric Analysis of the Effect of Actinomycin D, the PIH Analogue 2-Hydroxy-1-Naphthylaldehyde Isonicotinoyl Hydrazone (311), or DFO on Cell Cycle Distribution and Apoptosis in SK-N-MC, IMR-32, HL-60, and K562 Cells After 24 Hours of Incubation
| Treatments . | Cell Cycle Distribution . | % Debris . | % Apoptosis . | ||
|---|---|---|---|---|---|
| . | (% total) . | . | . | ||
| . | G0/G1 . | G2/M . | S . | . | . |
| SK-N-MC | |||||
| Control | 51 | 14 | 35 | 8 | 0 |
| Actin. D (1 μg/mL) | 95 | 0 | 5 | 28 | 52 |
| 311 (10 μmol/L) | 38 | 0 | 62 | 38 | 47 |
| DFO (2.5 mmol/L ) | 70 | 1 | 28 | 15 | 27 |
| IMR-32 | |||||
| Control | 38 | 8 | 54 | 9 | 0 |
| Actin. D (1 μg/mL) | 45 | 9 | 46 | 24 | 0 |
| 311 (10 μmol/L) | 45 | 8 | 47 | 26 | 0 |
| DFO (2.5 mmol/L ) | 58 | 0 | 42 | 31 | 0 |
| HL-60 | |||||
| Control | 48 | 1 | 51 | 1 | 0 |
| Actin. D (1 μg/mL) | 45 | 1 | 54 | 25 | 22 |
| 311 (10 μmol/L) | 56 | 23 | 21 | 42 | 0 |
| DFO (2.5 mmol/L ) | 69 | 0 | 31 | 13 | 12 |
| K562 | |||||
| Control | 33 | 28 | 39 | 4 | 0 |
| Actin. D (1 μg/mL) | 44 | 34 | 22 | 26 | 3 |
| 311 (10 μmol/L) | 51 | 41 | 8 | 2 | 0 |
| DFO (2.5 mmol/L ) | 65 | 27 | 8 | 7 | 0 |
| Treatments . | Cell Cycle Distribution . | % Debris . | % Apoptosis . | ||
|---|---|---|---|---|---|
| . | (% total) . | . | . | ||
| . | G0/G1 . | G2/M . | S . | . | . |
| SK-N-MC | |||||
| Control | 51 | 14 | 35 | 8 | 0 |
| Actin. D (1 μg/mL) | 95 | 0 | 5 | 28 | 52 |
| 311 (10 μmol/L) | 38 | 0 | 62 | 38 | 47 |
| DFO (2.5 mmol/L ) | 70 | 1 | 28 | 15 | 27 |
| IMR-32 | |||||
| Control | 38 | 8 | 54 | 9 | 0 |
| Actin. D (1 μg/mL) | 45 | 9 | 46 | 24 | 0 |
| 311 (10 μmol/L) | 45 | 8 | 47 | 26 | 0 |
| DFO (2.5 mmol/L ) | 58 | 0 | 42 | 31 | 0 |
| HL-60 | |||||
| Control | 48 | 1 | 51 | 1 | 0 |
| Actin. D (1 μg/mL) | 45 | 1 | 54 | 25 | 22 |
| 311 (10 μmol/L) | 56 | 23 | 21 | 42 | 0 |
| DFO (2.5 mmol/L ) | 69 | 0 | 31 | 13 | 12 |
| K562 | |||||
| Control | 33 | 28 | 39 | 4 | 0 |
| Actin. D (1 μg/mL) | 44 | 34 | 22 | 26 | 3 |
| 311 (10 μmol/L) | 51 | 41 | 8 | 2 | 0 |
| DFO (2.5 mmol/L ) | 65 | 27 | 8 | 7 | 0 |
Results are means of duplicate determinations in a typical experiment of two experiments performed.