Flow Cytometric Analysis Examining the Effect of the Concentration of DFO (10 μmol/L to 2.5 mmol/L) or the PIH Analogue 2-Hydroxy-1-Naphthylaldehyde Isocotinoyl Hydrazone (311; 0.1 to 10 μmol/L) on Cell Cycle Distribution and Apoptosis in HL-60 Leukemia Cells After 24 Hours of Incubation
| Treatments . | Cell Cycle Distribution . | % Debris . | % Apoptosis . | ||
|---|---|---|---|---|---|
| . | (% total) . | . | . | ||
| . | G0/G1 . | G2/M . | S . | . | . |
| Control | 46 | 0 | 54 | 2 | 0 |
| 10 μmol/L DFO | 46 | 7 | 47 | 1 | 0 |
| 100 μmol/L DFO | 48 | 0 | 52 | 5 | 0 |
| 500 μmol/L DFO | 75 | 0 | 25 | 26 | 0 |
| 2.5 mmol/L DFO | 81 | 0 | 19 | 26 | 7 |
| 0.1 μmol/L 311 | 46 | 3 | 50 | 2 | 0 |
| 1 μmol/L 311 | 45 | 7 | 48 | 2 | 0 |
| 5 μmol/L 311 | 44 | 8 | 47 | 7 | 31 |
| 10 μmol/L 311 | 50 | 0 | 50 | 35 | 25 |
| Actin. D (1 μg/mL) | 40 | 0 | 60 | 19 | 53 |
| Azide (0.05%) | 74 | 6 | 20 | 33 | 0 |
| Treatments . | Cell Cycle Distribution . | % Debris . | % Apoptosis . | ||
|---|---|---|---|---|---|
| . | (% total) . | . | . | ||
| . | G0/G1 . | G2/M . | S . | . | . |
| Control | 46 | 0 | 54 | 2 | 0 |
| 10 μmol/L DFO | 46 | 7 | 47 | 1 | 0 |
| 100 μmol/L DFO | 48 | 0 | 52 | 5 | 0 |
| 500 μmol/L DFO | 75 | 0 | 25 | 26 | 0 |
| 2.5 mmol/L DFO | 81 | 0 | 19 | 26 | 7 |
| 0.1 μmol/L 311 | 46 | 3 | 50 | 2 | 0 |
| 1 μmol/L 311 | 45 | 7 | 48 | 2 | 0 |
| 5 μmol/L 311 | 44 | 8 | 47 | 7 | 31 |
| 10 μmol/L 311 | 50 | 0 | 50 | 35 | 25 |
| Actin. D (1 μg/mL) | 40 | 0 | 60 | 19 | 53 |
| Azide (0.05%) | 74 | 6 | 20 | 33 | 0 |
Results are means of duplicate determinations in a typical experiment of three experiments performed.