The Effect of the PIH Analogues Compared With DFO on the Incorporation of 3H-Thymidine Over 24 and 48 Hours of Incubation by a Range of Human Neoplastic Cell Lines
| Cell Line . | Chelator . | 24 h . | 48 h . | ||
|---|---|---|---|---|---|
| . | . | IC50 ( μmol/L) . | IC90 ( μmol/L) . | IC50 ( μmol/L) . | IC90 ( μmol/L) . |
| SK-Mel-28 melanoma cells | DFO | 9.2 | 55.0 | 3.0 | 11.0 |
| 206 | 0.8 | 1.5 | 0.6 | 1.4 | |
| 308 | 2.7 | 5.7 | 1.9 | 3.0 | |
| 309 | 1.1 | 2.5 | 0.7 | 1.4 | |
| 311 | 0.9 | 2.6 | 0.5 | 1.1 | |
| 315 | 1.0 | 2.1 | 0.7 | 1.4 | |
| K562 erythroleukemia | DFO | 60 | 275 | 12.0 | 65 |
| 206 | 1.3 | 4.1 | 0.2 | 1.1 | |
| 308 | 6.4 | >12.5 | 2.4 | 5.3 | |
| 309 | 3.0 | 10.3 | 1.2 | 5.2 | |
| 311 | 0.7 | >12.5 | 0.1 | 0.3 | |
| 315 | 1.3 | 5.4 | 0.2 | 1.1 | |
| HL-60 leukemia | DFO | 35 | 125 | 15.0 | 35 |
| 206 | 0.4 | 1.0 | 0.2 | 0.4 | |
| 308 | 0.9 | 2.7 | 0.6 | 1.1 | |
| 309 | 0.7 | 1.9 | 0.5 | 0.8 | |
| 311 | 0.6 | 2.7 | 0.4 | 0.8 | |
| 315 | 0.6 | 1.3 | 0.3 | 0.5 | |
| LAI-55n NB | DFO | 5.0 | 12.0 | 1.7 | 26.0 |
| 206 | 0.8 | 1.9 | 0.6 | 0.8 | |
| 308 | 4.6 | 10.0 | 2.8 | 5.5 | |
| 309 | 2.3 | 5.7 | 1.2 | 2.0 | |
| 311 | 0.2 | 3.0 | 0.02 | 0.2 | |
| 315 | 0.7 | 2.7 | 0.5 | 0.7 | |
| IMR-32 NB | DFO | 3.0 | 24.0 | 1.2 | 5.0 |
| 206 | 0.4 | 0.7 | 0.3 | 0.7 | |
| 308 | 2.8 | 5.6 | 1.9 | 2.9 | |
| 309 | 1.0 | 2.5 | 0.6 | 1.3 | |
| 311 | 0.2 | 1.1 | 0.1 | 0.2 | |
| 315 | 0.5 | 1.3 | 0.2 | 0.5 | |
| CHP-234 NB | DFO | 5.0 | 20.5 | 1.5 | 6.7 |
| 206 | 0.7 | 1.5 | 0.4 | 1.0 | |
| 308 | 1.5 | 3.1 | 1.0 | 1.8 | |
| 309 | 1.8 | 4.2 | 1.3 | 2.7 | |
| 311 | 1.0 | 8.7 | 0.6 | 1.5 | |
| 315 | 0.7 | 2.0 | 0.5 | 0.9 | |
| SK-N-MC NB | DFO | 7.1 | 85 | 4.8 | 11.0 |
| 206 | 0.3 | 0.6 | 0.2 | 0.4 | |
| 308 | 0.6 | 1.3 | 0.3 | 0.6 | |
| 309 | 0.5 | 1.0 | 0.2 | 0.3 | |
| 311 | 0.5 | 0.8 | 0.2 | 0.3 | |
| 315 | 0.3 | 0.7 | 0.3 | 0.4 | |
| SK-N-BE NB | DFO | >400 | >400 | 280 | >400 |
| 206 | 1.0 | 3.9 | 0.2 | 0.4 | |
| 308 | 4.6 | 11.6 | 1.2 | 2.5 | |
| 309 | 2.5 | 5.6 | 0.6 | 1.3 | |
| 311 | 0.8 | 5.5 | 0.2 | 1.5 | |
| 315 | 1.2 | 3.4 | 0.3 | 0.6 | |
| Cell Line . | Chelator . | 24 h . | 48 h . | ||
|---|---|---|---|---|---|
| . | . | IC50 ( μmol/L) . | IC90 ( μmol/L) . | IC50 ( μmol/L) . | IC90 ( μmol/L) . |
| SK-Mel-28 melanoma cells | DFO | 9.2 | 55.0 | 3.0 | 11.0 |
| 206 | 0.8 | 1.5 | 0.6 | 1.4 | |
| 308 | 2.7 | 5.7 | 1.9 | 3.0 | |
| 309 | 1.1 | 2.5 | 0.7 | 1.4 | |
| 311 | 0.9 | 2.6 | 0.5 | 1.1 | |
| 315 | 1.0 | 2.1 | 0.7 | 1.4 | |
| K562 erythroleukemia | DFO | 60 | 275 | 12.0 | 65 |
| 206 | 1.3 | 4.1 | 0.2 | 1.1 | |
| 308 | 6.4 | >12.5 | 2.4 | 5.3 | |
| 309 | 3.0 | 10.3 | 1.2 | 5.2 | |
| 311 | 0.7 | >12.5 | 0.1 | 0.3 | |
| 315 | 1.3 | 5.4 | 0.2 | 1.1 | |
| HL-60 leukemia | DFO | 35 | 125 | 15.0 | 35 |
| 206 | 0.4 | 1.0 | 0.2 | 0.4 | |
| 308 | 0.9 | 2.7 | 0.6 | 1.1 | |
| 309 | 0.7 | 1.9 | 0.5 | 0.8 | |
| 311 | 0.6 | 2.7 | 0.4 | 0.8 | |
| 315 | 0.6 | 1.3 | 0.3 | 0.5 | |
| LAI-55n NB | DFO | 5.0 | 12.0 | 1.7 | 26.0 |
| 206 | 0.8 | 1.9 | 0.6 | 0.8 | |
| 308 | 4.6 | 10.0 | 2.8 | 5.5 | |
| 309 | 2.3 | 5.7 | 1.2 | 2.0 | |
| 311 | 0.2 | 3.0 | 0.02 | 0.2 | |
| 315 | 0.7 | 2.7 | 0.5 | 0.7 | |
| IMR-32 NB | DFO | 3.0 | 24.0 | 1.2 | 5.0 |
| 206 | 0.4 | 0.7 | 0.3 | 0.7 | |
| 308 | 2.8 | 5.6 | 1.9 | 2.9 | |
| 309 | 1.0 | 2.5 | 0.6 | 1.3 | |
| 311 | 0.2 | 1.1 | 0.1 | 0.2 | |
| 315 | 0.5 | 1.3 | 0.2 | 0.5 | |
| CHP-234 NB | DFO | 5.0 | 20.5 | 1.5 | 6.7 |
| 206 | 0.7 | 1.5 | 0.4 | 1.0 | |
| 308 | 1.5 | 3.1 | 1.0 | 1.8 | |
| 309 | 1.8 | 4.2 | 1.3 | 2.7 | |
| 311 | 1.0 | 8.7 | 0.6 | 1.5 | |
| 315 | 0.7 | 2.0 | 0.5 | 0.9 | |
| SK-N-MC NB | DFO | 7.1 | 85 | 4.8 | 11.0 |
| 206 | 0.3 | 0.6 | 0.2 | 0.4 | |
| 308 | 0.6 | 1.3 | 0.3 | 0.6 | |
| 309 | 0.5 | 1.0 | 0.2 | 0.3 | |
| 311 | 0.5 | 0.8 | 0.2 | 0.3 | |
| 315 | 0.3 | 0.7 | 0.3 | 0.4 | |
| SK-N-BE NB | DFO | >400 | >400 | 280 | >400 |
| 206 | 1.0 | 3.9 | 0.2 | 0.4 | |
| 308 | 4.6 | 11.6 | 1.2 | 2.5 | |
| 309 | 2.5 | 5.6 | 0.6 | 1.3 | |
| 311 | 0.8 | 5.5 | 0.2 | 1.5 | |
| 315 | 1.2 | 3.4 | 0.3 | 0.6 | |
Results are means from quadruplicate determinations.