CD77 and CD44 Expression by GC B Cells Maintained in the CD40 Culture System
| Cytokines . | Experiment 1 . | Experiment 2 . | ||
|---|---|---|---|---|
| . | %CD38+CD77+ . | %CD38+CD44− . | %CD38+CD77+ . | %CD38+CD44− . |
| Day 0 | 72 | 61 | 79 | 63 |
| IL-10 + IL-1β + IL-3 | 31 | 23 | 34 | 24 |
| IL-10 + IL-1β + IL-2 | 24 | 33 | 30 | 40 |
| IL-10 + IL-3 + IL-7 | 26 | 20 | 34 | 28 |
| IL-10 + IL-4 + IL-7 | 18 | 25 | 26 | 35 |
| IL-10 + IL-3 + IL-6 | 26 | 20 | 33 | 25 |
| Cytokines . | Experiment 1 . | Experiment 2 . | ||
|---|---|---|---|---|
| . | %CD38+CD77+ . | %CD38+CD44− . | %CD38+CD77+ . | %CD38+CD44− . |
| Day 0 | 72 | 61 | 79 | 63 |
| IL-10 + IL-1β + IL-3 | 31 | 23 | 34 | 24 |
| IL-10 + IL-1β + IL-2 | 24 | 33 | 30 | 40 |
| IL-10 + IL-3 + IL-7 | 26 | 20 | 34 | 28 |
| IL-10 + IL-4 + IL-7 | 18 | 25 | 26 | 35 |
| IL-10 + IL-3 + IL-6 | 26 | 20 | 33 | 25 |
GC B cells were cultured for 8 days with CD40 MoAb, G28-5 in the presence of CD32-L cells and various combinations of 3 cytokines. Cells recovered were >93% CD38+, >95% CD19+, and <1% CD3+.