Adhesion of Clonogenic CD34+ Progenitors to HA
| HA-Adherent CD34+ Cells . | CFU-GM (%) . | BFU-E (%) . |
|---|---|---|
| Spontaneous | 19.6 ± 2.5 | 7.3 ± 1.0 |
| H90-induced | 21.3 ± 2.8 | 34.0 ± 2.5 |
| SCF-induced | 53.3 ± 5.5* | 7.2 ± 2.0† |
| IL-3–induced | 49.5 ± 7.2* | 10.0 ± 4.3† |
| GM-CSF–induced* | 56.6 ± 6.2* | 6.1 ± 3.0† |
| HA-Adherent CD34+ Cells . | CFU-GM (%) . | BFU-E (%) . |
|---|---|---|
| Spontaneous | 19.6 ± 2.5 | 7.3 ± 1.0 |
| H90-induced | 21.3 ± 2.8 | 34.0 ± 2.5 |
| SCF-induced | 53.3 ± 5.5* | 7.2 ± 2.0† |
| IL-3–induced | 49.5 ± 7.2* | 10.0 ± 4.3† |
| GM-CSF–induced* | 56.6 ± 6.2* | 6.1 ± 3.0† |
CD34+ cells, suspended at 2 × 104 cells/mL cell adhesion medium, were incubated at 4°C for 15 minutes with 5 μg/mL IgG1 or H90 MoAb. Aliquots (100 μL) were seeded in triplicate into HA-coated wells, and, when indicated, GM-CSF, IL-3, or SCF (10 ng/mL) were directly added into the wells. After incubation of 37°C for 15 minutes in contact to immobilized HA, nonadherent cells were removed as described in the Materials and Methods. Adherent cells were seeded in a complete methylcellulose medium supplemented with stimulating factors, as described, and CFU-GM and BFU-E were scored at day 16 (short-term methylcellulose assay). Results were expressed as the percentage of colonies obtained in the input (same cells but without adhesion step), as determined in a parallel experiment. The 100% corresponded to an input of 294 ± 15 CFU, comprising 199 ± 20 BFU-E and 95 ± 10 CFU-GM. All cultures were made in triplicate. Data are the means ± SE of three independent experiments.
P < .05 compared with control (spontaneous).
P > .05 compared with control (spontaneous).