Primers Used for Amplification of HTLV-1
| Region . | Primer Nucleotide Sequence . | Product Size . |
|---|---|---|
| gag | sense 5′-TATGCAGACCATCCGGCTTG-3′ (1324-1343) | |
| anti-sense 5′-TTGTTGGCTTGGACACGGAG-3′ (1521-1502) | 198 bp | |
| pol | sense 5′-AGGCCTTGCAACACTTGGTC-3′ (2631-2650) | |
| anti-sense 5′-GGATGAATCGCCAGGTTCCA-3′ (2750-2731) | 120 bp | |
| env | sense 5′-TCGACGCTCCAGGATATGAC-3′ (5696-5715) | |
| anti-sense 5′-GAGGCTGGCACGATCGATAC-3′ (5904-5885) | 209 bp | |
| pX | sense 5′-GGCTCCGTTGTCCGCATGTA-3′ (7764-7783) | |
| anti-sense 5′-AATCATAGGCGTGCCATCGG-3′ (8090-8071) | 327 bp |
| Region . | Primer Nucleotide Sequence . | Product Size . |
|---|---|---|
| gag | sense 5′-TATGCAGACCATCCGGCTTG-3′ (1324-1343) | |
| anti-sense 5′-TTGTTGGCTTGGACACGGAG-3′ (1521-1502) | 198 bp | |
| pol | sense 5′-AGGCCTTGCAACACTTGGTC-3′ (2631-2650) | |
| anti-sense 5′-GGATGAATCGCCAGGTTCCA-3′ (2750-2731) | 120 bp | |
| env | sense 5′-TCGACGCTCCAGGATATGAC-3′ (5696-5715) | |
| anti-sense 5′-GAGGCTGGCACGATCGATAC-3′ (5904-5885) | 209 bp | |
| pX | sense 5′-GGCTCCGTTGTCCGCATGTA-3′ (7764-7783) | |
| anti-sense 5′-AATCATAGGCGTGCCATCGG-3′ (8090-8071) | 327 bp |
Complete proviral sequences is described elsewhere.25