Recognition of MHC Class II Determinants by Autocytotoxic T Cells Associated With Autologous GVHD
| Target Cells . | Percentage of Specific Lysis of Treated Target Cells Mediated by Autologous GVHD Effector Lymphocytes . | |||||
|---|---|---|---|---|---|---|
| . | Experiment No. . | Pretreatment of Target Cells . | . | . | ||
| . | . | Normal Mouse Serum . | Anti-MHC Class I . | Anti-MHC Class II . | . | . |
| Autologous lymphoblasts | 1 | 43.7 | 40.6 | 10.5 | ||
| 2 | 23.7 | 21.3 | 5.4 | |||
| T47D | 1 | 28.7 | 29.6 | 4.9 | ||
| 2 | 48.2 | NT | 8.3 | |||
| Target Cells . | Percentage of Specific Lysis of Treated Target Cells Mediated by Autologous GVHD Effector Lymphocytes . | |||||
|---|---|---|---|---|---|---|
| . | Experiment No. . | Pretreatment of Target Cells . | . | . | ||
| . | . | Normal Mouse Serum . | Anti-MHC Class I . | Anti-MHC Class II . | . | . |
| Autologous lymphoblasts | 1 | 43.7 | 40.6 | 10.5 | ||
| 2 | 23.7 | 21.3 | 5.4 | |||
| T47D | 1 | 28.7 | 29.6 | 4.9 | ||
| 2 | 48.2 | NT | 8.3 | |||
Autologous pretransplant lymphoblasts and T47D cells were labeled with 51Cr and pretreated with either normal mouse serum or murine MoAbs to the MHC class I and MHC class II determinants for 1 hour at 4°C. The cells were washed in RPMI 1640 before use as targets in the 51Cr release assay. Effector T cells were derived from patients with biopsy confirmed autologous GVHD. The effector to target ratio was 100:1.
Abbreviation: NT, not tested.