Table 3. Platelet Adhesion to Collagen... | American Society of Hematology

Table 3.

Platelet Adhesion to Collagen Type III of vWF; Addition of the Recombinant vWFs to Normal Whole Blood

Surface	Addition	Anticoagulant	Coverage as % of Control
CoII II	WT	Citrate	100
	R543Q		19 ± 7^3-150
	R543W		15 ± 7^3-150
CoII III	WT	LMWH	100
	R543Q		50 ± 8^3-151
	R543W		73 ± 5^3-151
vWF	WT	Citrate	100
	R543Q		102 ± 7
	R543W		98 ± 3

Surface	Addition	Anticoagulant	Coverage as % of Control
CoII II	WT	Citrate	100
	R543Q		19 ± 7^3-150
	R543W		15 ± 7^3-150
CoII III	WT	LMWH	100
	R543Q		50 ± 8^3-151
	R543W		73 ± 5^3-151
vWF	WT	Citrate	100
	R543Q		102 ± 7
	R543W		98 ± 3

Recombinant vWFs (WT, R543Q, and R543W) were added to citrated or LMWH anticoagulated whole blood at 0.5 μg/mL and perfusion was performed using collagen type III or vWF-coated coverslips. The coverslips were exposed to shear rate 1,600 s⁻¹ for 5 minutes at 37°C. The data are mean ± SEM obtained in three independent experiments each performed in triplicate. The mean platelet coverage on CoII III in citrate for WT is 19%, on CoII III in LMWH is 42.3%, and on vWF 56.7%. The platelet count of the perfusates does not differ more than 10%.

F3-150

P < .01.

F3-151

P < .05.

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