Platelet Adhesion to Recombinant vWFs Under Flow Conditions
| Surface . | % Platelet Coverage . | |
|---|---|---|
| . | 300 s−1 . | 1,600 s−1 . |
| WT | 37 ± 10 | 48 ± 10 |
| R543Q | 41 ± 2 (NS) | 49 ± 6 (NS) |
| R543W | 28 ± 7 (NS) | 34 ± 6 (NS) |
| HAS | 1.1 ± 0.5* | 0.9 ± 0.5* |
| Surface . | % Platelet Coverage . | |
|---|---|---|
| . | 300 s−1 . | 1,600 s−1 . |
| WT | 37 ± 10 | 48 ± 10 |
| R543Q | 41 ± 2 (NS) | 49 ± 6 (NS) |
| R543W | 28 ± 7 (NS) | 34 ± 6 (NS) |
| HAS | 1.1 ± 0.5* | 0.9 ± 0.5* |
Recombinant vWFs (WT, R543Q, and R543W) (10 μg/mL) were coated to glass coverslips. As a control, human albumin (HAS) was coated. The coverslips were exposed to two shear rates: 300 s−1 and 1,600 s−1 using citrated whole blood as the perfusate. The data are mean ± SEM obtained in six independent experiments each performed in triplicate.
Abbreviation: NS, not significant.
P < .001 compared with WT-vWF.