PCR primers |
1. GACCTTGTAAAGTCAAAAATGTC |
2. bio CTCTCTACAATGCAACCCATG |
3. bio CTTGTGAAATCAACCTAACAGAG |
4. CTGACCATAGCTCCAGCTTC |
5. GAAGATGACCTGCCCACAGTG |
6. bio CTCTGCAAAAACAAAAGGTGC |
7. bio ACCATCCAGGTGTACCCAGAC |
Cloning primers |
5restr. TTCAAGCTTGAAGATGACCTGCCCACAGTG Hind III |
7restr. ATATCTAGAACCATCCAGGTGTACCCAGAC Xba I |
Minisequencing primers |
T → C intron C TGGAATACGTCATTGGTGAG |
Arg661 → Stop (C → T) ATCCTTTAAAAGAAACCCTG |
PCR primers |
1. GACCTTGTAAAGTCAAAAATGTC |
2. bio CTCTCTACAATGCAACCCATG |
3. bio CTTGTGAAATCAACCTAACAGAG |
4. CTGACCATAGCTCCAGCTTC |
5. GAAGATGACCTGCCCACAGTG |
6. bio CTCTGCAAAAACAAAAGGTGC |
7. bio ACCATCCAGGTGTACCCAGAC |
Cloning primers |
5restr. TTCAAGCTTGAAGATGACCTGCCCACAGTG Hind III |
7restr. ATATCTAGAACCATCCAGGTGTACCCAGAC Xba I |
Minisequencing primers |
T → C intron C TGGAATACGTCATTGGTGAG |
Arg661 → Stop (C → T) ATCCTTTAAAAGAAACCCTG |
Abbreviation: bio, biotinylated primer; restr, primer with restriction enzyme site.