Percent Apoptosis of Human NK Cells
| Donor No. . | IL-2 . | IL-2 + IL-12* . | Donor No. . | IL-15 . | IL-15 + IL-12* . |
|---|---|---|---|---|---|
| 1 | 5 | 36 | 10 | 1 | 39 |
| 2 | 8 | 63 | 11 | 10 | 50 |
| 3 | 19 | 40 | 12 | 10 | 63 |
| 4 | 20 | 74 | 13 | 10 | 70 |
| 5 | 23 | 58 | 14 | 18 | 62 |
| 6 | 16 | 69 | 15 | 8 | 67 |
| 7 | 18 | 53 | 16 | 19 | 52 |
| 8 | 21 | 48 | 17 | 20 | 49 |
| 9 | 28 | 63 | 18 | 23 | 64 |
| Donor No. . | IL-2 . | IL-2 + IL-12* . | Donor No. . | IL-15 . | IL-15 + IL-12* . |
|---|---|---|---|---|---|
| 1 | 5 | 36 | 10 | 1 | 39 |
| 2 | 8 | 63 | 11 | 10 | 50 |
| 3 | 19 | 40 | 12 | 10 | 63 |
| 4 | 20 | 74 | 13 | 10 | 70 |
| 5 | 23 | 58 | 14 | 18 | 62 |
| 6 | 16 | 69 | 15 | 8 | 67 |
| 7 | 18 | 53 | 16 | 19 | 52 |
| 8 | 21 | 48 | 17 | 20 | 49 |
| 9 | 28 | 63 | 18 | 23 | 64 |
Purified NK cells were cultured for 3 days with IL-2 (15 ng/mL) or IL-2 plus IL-12 (3 ng/mL); IL-15 (15 ng/mL) or IL-15 plus IL-12. The percentage of cells undergoing apoptosis was determined by nuclear staining with PI using flow cytometry. Results from 20 individual donors are shown. The standard deviation of the mean for experiments done in triplicate (donors 6 to 9 and 15 to 18) was <8%.
P = .004 as calculated by the Exact Wilcoxon Signed Rank Test.