Myeloid and Erythroid Progenitor Activity Detected Among Populations of CD4+ and CD4− Fetal Liver Progenitors
| Experiment . | Cell Population* . | HPP-CFC† . | LPP-CFC† . | BFU-E† . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | CD4+ . | CD4− . | P‡ . | CD4+ . | CD4− . | P‡ . | CD4+ . | CD4− . | P‡ . |
| A | CD34++ Lin− | 32.0 ± 2.0 | 14.2 ± 2.5 | .0080 | 52.9 ± 0.9 | 40.9 ± 3.2 | .0460 | 2.7 ± 0.8 | 6.7 ± 0.8 | .0351 |
| B | CD34++ Lin− | 96.9 ± 1.6 | 58.7 ± 10.1 | .0712 | 67.1 ± 8.6 | 75.6 ± 7.6 | .0762 | 6.7 ± 1.5 | 25.8 ± 0.4 | .0101 |
| C | CD34++ Lin− | 82.2 ± 3.5 | 45.3 ± 5.4 | .0237 | 79.6 ± 8.0 | 98.7 ± 7.1 | .1439 | ND | ND | ND |
| D | CD34++ Lin− | 78.2 ± 6.5 | 28.4 ± 3.8 | .0100 | 54.2 ± 6.2 | 87.6 ± 12.0 | .0325 | ND | ND | ND |
| E | CD34++ Lin− | 124.4 ± 4.5 | 52.9 ± 2.7 | .0042 | 149.3 ± 8.0 | 166.2 ± 7.5 | .3844 | 20.0 ± 6.7 | 44.9 ± 2.4 | .0441 |
| F | CD34++ Lin− | 96.0 ± 4.2 | 74.7 ± 7.4 | .0343 | 170.7 ± 2.9 | 130 ± 3.1 | .0035 | 23.1 ± 4.2 | 48.4 ± 11.6 | .1013 |
| G | CD34++ CD38− Lin− | 117.8 ± 13.5 | 57.8 ± 9.5 | .0574 | 158.9 ± 15.4 | 95.6 ± 7.3 | .0747 | ND | ND | ND |
| H | CD34++ CD38− Lin− | 284.4 ± 33.2 | 90.4 ± 15.5 | .0230 | 87.8 ± 15.4 | 171.9 ± 10.4 | .0080 | ND | ND | ND |
| I | CD34++ CD38− Lin− | 173.3 ± 5.8 | 125.6 ± 9.5 | .0148 | 255.6 ± 4.0 | 217.8 ± 13.5 | .0984 | ND | ND | ND |
| Experiment . | Cell Population* . | HPP-CFC† . | LPP-CFC† . | BFU-E† . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | CD4+ . | CD4− . | P‡ . | CD4+ . | CD4− . | P‡ . | CD4+ . | CD4− . | P‡ . |
| A | CD34++ Lin− | 32.0 ± 2.0 | 14.2 ± 2.5 | .0080 | 52.9 ± 0.9 | 40.9 ± 3.2 | .0460 | 2.7 ± 0.8 | 6.7 ± 0.8 | .0351 |
| B | CD34++ Lin− | 96.9 ± 1.6 | 58.7 ± 10.1 | .0712 | 67.1 ± 8.6 | 75.6 ± 7.6 | .0762 | 6.7 ± 1.5 | 25.8 ± 0.4 | .0101 |
| C | CD34++ Lin− | 82.2 ± 3.5 | 45.3 ± 5.4 | .0237 | 79.6 ± 8.0 | 98.7 ± 7.1 | .1439 | ND | ND | ND |
| D | CD34++ Lin− | 78.2 ± 6.5 | 28.4 ± 3.8 | .0100 | 54.2 ± 6.2 | 87.6 ± 12.0 | .0325 | ND | ND | ND |
| E | CD34++ Lin− | 124.4 ± 4.5 | 52.9 ± 2.7 | .0042 | 149.3 ± 8.0 | 166.2 ± 7.5 | .3844 | 20.0 ± 6.7 | 44.9 ± 2.4 | .0441 |
| F | CD34++ Lin− | 96.0 ± 4.2 | 74.7 ± 7.4 | .0343 | 170.7 ± 2.9 | 130 ± 3.1 | .0035 | 23.1 ± 4.2 | 48.4 ± 11.6 | .1013 |
| G | CD34++ CD38− Lin− | 117.8 ± 13.5 | 57.8 ± 9.5 | .0574 | 158.9 ± 15.4 | 95.6 ± 7.3 | .0747 | ND | ND | ND |
| H | CD34++ CD38− Lin− | 284.4 ± 33.2 | 90.4 ± 15.5 | .0230 | 87.8 ± 15.4 | 171.9 ± 10.4 | .0080 | ND | ND | ND |
| I | CD34++ CD38− Lin− | 173.3 ± 5.8 | 125.6 ± 9.5 | .0148 | 255.6 ± 4.0 | 217.8 ± 13.5 | .0984 | ND | ND | ND |
Abbreviation: ND, not determined.
CD4+ CD34++ Lin− and CD4− CD34++ Lin− LDFL cells were isolated by immunomagnetic bead depletion of GPA− LDFL cells using a cocktail of PE-labeled MoAbs against CD3, CD8, CD10, CD14, CD16, CD19, CD20, and CD56. These Lin− cells were then stained for CD34 and CD4 expression using anti-CD34-biotin followed by streptavidin-APC and anti-CD4-FITC. Electronic gates for FACS were defined as shown in Fig 1. CD4+ CD34++ CD38− Lin− and CD4− CD34++ CD38− Lin− LDFL cells were isolated using a similar protocol except that the cocktail of MoAbs against Lin markers was FITC-labeled and did not contain anti-CD56 but did contain anti-CD38. Consequently, CD4 expression was detected using anti-CD4-PE.
Results are presented as the mean CFU-C ± SE/1 × 103 cells. Triplicate cultures of CD4+ CD34++ Lin− or CD4− CD34++ Lin− LDFL cells were initiated with 7.5 × 102 cells/culture dish. Cultures of CD4+ CD34++ CD38− Lin− or CD4− CD34++ CD38− Lin− LDFL cells were initiated with 2.0 to 3.0 × 102 cells/culture dish.
P values were determined using the Student's t-test.