Table 1.

Degree of Resistance to Cytotoxic Drugs and Fas-Mediated Apoptosis

Cell LineIC50 of Selecting Agent (mol/L)DOR to% Fas-Induced% Decrease
DrugCell Deathin Apoptosis
8226/S Dox 2.5 × 10−9 1 62  
  MR 9.7 × 10−8 
8226/Dox1 3.2 × 10−8 13 49 21 
8226/Dox6 2.8 × 10−7 110 21 66 
8226/Dox40 2.7 × 10−6 1,350 12 81 
8226/Dox40ood 4.5 × 10−7 180 18 71 
8226/MR4 1.0 × 10−6 11 42 32 
8226/MR20 3.6 × 10−6 37 19 69 
8226/MR20ood 2.8 × 10−6 30 21 66 
CEM/S 3.2 × 10−8 1 96  
CEM/Dox1-F5 2.9 × 10−9 3 72 25 
Cell LineIC50 of Selecting Agent (mol/L)DOR to% Fas-Induced% Decrease
DrugCell Deathin Apoptosis
8226/S Dox 2.5 × 10−9 1 62  
  MR 9.7 × 10−8 
8226/Dox1 3.2 × 10−8 13 49 21 
8226/Dox6 2.8 × 10−7 110 21 66 
8226/Dox40 2.7 × 10−6 1,350 12 81 
8226/Dox40ood 4.5 × 10−7 180 18 71 
8226/MR4 1.0 × 10−6 11 42 32 
8226/MR20 3.6 × 10−6 37 19 69 
8226/MR20ood 2.8 × 10−6 30 21 66 
CEM/S 3.2 × 10−8 1 96  
CEM/Dox1-F5 2.9 × 10−9 3 72 25 
Degree of resistance (DOR) is relative to the parent cell line, 8226/S. The DOR is determined by dividing the IC50 for the resistant cell line (8226/Dox, 8226/MR, or CEM/Dox) by the IC50 for the parent cell line.35 Fas-induced cell death is calculated by the percentage of apoptotic cells after treatment with 200 ng/mL of anti-Fas (CH-11) antibody minus the percentage of apoptotic cells in controls treated with mouse IgM. The percentage of decrease in apoptosis due to Fas is determined by
(%Cell Death in Sensitive Cell Line)−(%Cell Death in Resistant Cell Line)(%Cell Death in Sensitive Cell Line)
Each value represents the average of at least three independent trials.
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