Effect of CD2 Ligation on Some T-Cell Activation Markers
| . | Cell No. (×105/mL) . | CPM (×10−3) . | IL-2R+ (%) . | IL-2 (pg/mL) . |
|---|---|---|---|---|
| Medium | 19 ± 0.7 | 32 ± 3 | 2.7 ± 0.5 | 13 ± 0.3 |
| Anti-CD3 (1 μg) | 10 ± 0.5 | 19 ± 2 | 16.2 ± 3 | 489 ± 4 |
| Anti-CD2 (5 μg) | 20 ± 0.8 | 33 ± 1 | 1.8 ± 1 | 15 ± 0.5 |
| Anti-CD3 + anti-CD2 | 16.8 ± 0.4* | 26 ± 0.8* | 5.8 ± 1.7* | 410 ± 5* |
| . | Cell No. (×105/mL) . | CPM (×10−3) . | IL-2R+ (%) . | IL-2 (pg/mL) . |
|---|---|---|---|---|
| Medium | 19 ± 0.7 | 32 ± 3 | 2.7 ± 0.5 | 13 ± 0.3 |
| Anti-CD3 (1 μg) | 10 ± 0.5 | 19 ± 2 | 16.2 ± 3 | 489 ± 4 |
| Anti-CD2 (5 μg) | 20 ± 0.8 | 33 ± 1 | 1.8 ± 1 | 15 ± 0.5 |
| Anti-CD3 + anti-CD2 | 16.8 ± 0.4* | 26 ± 0.8* | 5.8 ± 1.7* | 410 ± 5* |
Data are means ± SD of three experiments. Cell count using trypan blue exclusion, [3H]thymidine uptake, IL-2 detection by ELISA, and IL-2R staining were performed after incubating 3DO cells for 20 hours.
P < .01 comparing line 4 with line 2.