Effects of Various Inhibitors on Aggregation and Tyrosine Phosphorylation of the 64-kD Protein Induced by NNKY5-5
| Agent . | Aggregation . | 64-kD Protein Phosphorylation . |
|---|---|---|
| Aspirin (500 μmol/L) | + | + |
| H-7 (100 μmol/L) | + | + |
| EGTA (3 mmol/L) | − | + |
| GRGDS (400 μmol/L) | − | + |
| Prostaglandin I2 (0.2 μmol/L) | − | − |
| Tyrphostin (100 μmol/L) | − | − |
| Herbimycin (25 μmol/L) | − | − |
| Agent . | Aggregation . | 64-kD Protein Phosphorylation . |
|---|---|---|
| Aspirin (500 μmol/L) | + | + |
| H-7 (100 μmol/L) | + | + |
| EGTA (3 mmol/L) | − | + |
| GRGDS (400 μmol/L) | − | + |
| Prostaglandin I2 (0.2 μmol/L) | − | − |
| Tyrphostin (100 μmol/L) | − | − |
| Herbimycin (25 μmol/L) | − | − |
Inhibitors were added to PRP 10 minutes before stimulation with 10 μg/mL NNKY5-5 and protein phosphorylation was assessed using gel-filtered platelets. The data were compiled from two experiments. Inhibition was judged to be negative when its magnitude was less than 30% on densitometry. PGI2 , tyrphostin, and herbmycin all caused almost complete inhibition of protein phosphorylation.
−, inhibited; +, not inhibited.