Numbers of Colonies Containing Erythroid Cells From Day 10.5 Yolk Sac Cells
| Factors . | Macroscopic Erythroid Colonies . | Microscopic Erythroid Colonies . | Percentage of Macroscopic/Microscopic . |
|---|---|---|---|
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| EPO | 1 ± 1 | 39 ± 3 | 4 ± 2 |
| TPO | 0 ± 0 | 12 ± 3 | 0 ± 0 |
| EPO + TPO | 34 ± 4* | 141 ± 12* | 24 ± 3* |
| IL-3 | 15 ± 2† | 93 ± 7† | 16 ± 2† |
| IL-3 + EPO | 94 ± 8‡ | 149 ± 13 | 63 ± 2‡ |
| IL-3 + TPO | 35 ± 5 | 134 ± 4 | 26 ± 3 |
| IL-3 + EPO + TPO | 152 ± 3‡ | 236 ± 11‡ | 65 ± 1‡ |
| Factors . | Macroscopic Erythroid Colonies . | Microscopic Erythroid Colonies . | Percentage of Macroscopic/Microscopic . |
|---|---|---|---|
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| EPO | 1 ± 1 | 39 ± 3 | 4 ± 2 |
| TPO | 0 ± 0 | 12 ± 3 | 0 ± 0 |
| EPO + TPO | 34 ± 4* | 141 ± 12* | 24 ± 3* |
| IL-3 | 15 ± 2† | 93 ± 7† | 16 ± 2† |
| IL-3 + EPO | 94 ± 8‡ | 149 ± 13 | 63 ± 2‡ |
| IL-3 + TPO | 35 ± 5 | 134 ± 4 | 26 ± 3 |
| IL-3 + EPO + TPO | 152 ± 3‡ | 236 ± 11‡ | 65 ± 1‡ |
A total of 7.5 × 105 day 10.5 yolk sac cells were plated per dish in the presence of EPO, TPO, IL-3, and their combinations. Data represent mean ± SE of quadruplicate cultures.
P < .01 by Student's t-test with the groups of None, EPO alone, and TPO alone.
P < .01 by Student's t-test with the group of TPO alone.
P < .01 by Student's t-test with the group of EPO + TPO.