PBMCs Express Soluble TNF in Response to LPS and IR
| Treatment . | TNF-α (pg/mL) . | ||
|---|---|---|---|
| . | 2 h . | 24 h . | 48 h . |
| Control | <4 | <4 | <4 |
| IR | 8 | 31 | 15 |
| LPS | 1,402 | 3,258 | 1,870 |
| Treatment . | TNF-α (pg/mL) . | ||
|---|---|---|---|
| . | 2 h . | 24 h . | 48 h . |
| Control | <4 | <4 | <4 |
| IR | 8 | 31 | 15 |
| LPS | 1,402 | 3,258 | 1,870 |
Supernatants of PBMCs either untreated (Control), irradiated (IR), or incubated in the presence of LPS were analyzed 2, 24, and 48 hours after treatment for soluble TNF-α (in picrograms per milliliter) by the ELISA sandwich technique as described in the Materials and Methods. These results are representative of three independent experiments.