Effect of hGATA-1, hGATA-2, and hGATA-3 Transcription Factors on Induction of the Wild-Type and the Mutated GATA of the Epo Promoter With Luciferase Reporter in Hep3B Cells
| No. . | Plasmid . | O2 . | Total RLU/CAT(U) . | |||
|---|---|---|---|---|---|---|
| . | . | (%) . | Exp 1 . | Fold Induction . | Exp 2 . | Fold Induction . |
| 1 | Pwt | 21 | 122.6 | 245.4 | ||
| 2 | Pwt | 1 | 12940.9 | 105.6 | 11401.3 | 46.5 |
| 3 | Pwt + hGATA-1 | 21 | 118.1 | 323.8 | ||
| 4 | Pwt + hGATA-1 | 1 | 1636.9 | 13.9 | 4253.1 | 13.1 |
| 5 | Pwt + hGATA-2 | 21 | 120.8 | 597.6 | ||
| 6 | Pwt + hGATA-2 | 1 | 5731.6 | 47.4 | 9305.0 | 15.6 |
| 7 | Pwt + hGATA-3 | 21 | 74.3 | 327.3 | ||
| 8 | Pwt + hGATA-3 | 1 | 3325.3 | 44.8 | 2934.9 | 9.0 |
| 9 | Pm7 | 21 | 119.8 | 661.4 | ||
| 10 | Pm7 | 1 | 14662.7 | 122.4 | 51381.9 | 77.7 |
| 11 | Pm7 + hGATA-1 | 21 | 74.9 | 405.2 | ||
| 12 | Pm7 + hGATA-1 | 1 | 16171.4 | 215.9 | 19940.5 | 49.2 |
| 13 | Pm7 + hGATA-2 | 21 | 189.5 | 486.0 | ||
| 14 | Pm7 + hGATA-2 | 1 | 49679.2 | 262.2 | 47970.2 | 98.7 |
| 15 | Pm7 + hGATA-3 | 21 | 96.1 | 315.4 | ||
| 16 | Pm7 + hGATA-3 | 1 | 34615.4 | 360.2 | 74370.6 | 235.8 |
| No. . | Plasmid . | O2 . | Total RLU/CAT(U) . | |||
|---|---|---|---|---|---|---|
| . | . | (%) . | Exp 1 . | Fold Induction . | Exp 2 . | Fold Induction . |
| 1 | Pwt | 21 | 122.6 | 245.4 | ||
| 2 | Pwt | 1 | 12940.9 | 105.6 | 11401.3 | 46.5 |
| 3 | Pwt + hGATA-1 | 21 | 118.1 | 323.8 | ||
| 4 | Pwt + hGATA-1 | 1 | 1636.9 | 13.9 | 4253.1 | 13.1 |
| 5 | Pwt + hGATA-2 | 21 | 120.8 | 597.6 | ||
| 6 | Pwt + hGATA-2 | 1 | 5731.6 | 47.4 | 9305.0 | 15.6 |
| 7 | Pwt + hGATA-3 | 21 | 74.3 | 327.3 | ||
| 8 | Pwt + hGATA-3 | 1 | 3325.3 | 44.8 | 2934.9 | 9.0 |
| 9 | Pm7 | 21 | 119.8 | 661.4 | ||
| 10 | Pm7 | 1 | 14662.7 | 122.4 | 51381.9 | 77.7 |
| 11 | Pm7 + hGATA-1 | 21 | 74.9 | 405.2 | ||
| 12 | Pm7 + hGATA-1 | 1 | 16171.4 | 215.9 | 19940.5 | 49.2 |
| 13 | Pm7 + hGATA-2 | 21 | 189.5 | 486.0 | ||
| 14 | Pm7 + hGATA-2 | 1 | 49679.2 | 262.2 | 47970.2 | 98.7 |
| 15 | Pm7 + hGATA-3 | 21 | 96.1 | 315.4 | ||
| 16 | Pm7 + hGATA-3 | 1 | 34615.4 | 360.2 | 74370.6 | 235.8 |
Two separate experiments were performed (n = 2). The luciferase activities observed in two experiments with hypoxia treatment with Pwt were 1.96 and 5.05 × 105 relative light units per 800 μL of extract, respectively.