Table 1. Phenotypic Analysis of CD19... | American Society of Hematology

Table 1.

Phenotypic Analysis of CD19⁺ Cells Produced by Human CD34⁺CD38^lowCD19⁻ and CD34⁺CD38^lowCD19⁻CD10⁻ Cord Blood Cells Cultured for 6 Weeks on the Murine Stromal Cells MS-5

Cell Fraction	No. of Experiments	Conditions	% CD19⁺	% of CD19⁺ Cells Expressing
				CD34	CD38	CD10	CD20	CD22
CD34⁺38^low19⁻	10	Lymphoid	43 ± 5.4	<1	100	100	4 ± 1	<1
	8	Switch	8.4 ± 3.6	<1	100	100	1.3 ± 0.3	<1
CD34⁺38^low19⁻10⁻	4	Lymphoid	30 ± 11	0	100	100	6.3 ± 3	0

Cell Fraction	No. of Experiments	Conditions	% CD19⁺	% of CD19⁺ Cells Expressing
				CD34	CD38	CD10	CD20	CD22
CD34⁺38^low19⁻	10	Lymphoid	43 ± 5.4	<1	100	100	4 ± 1	<1
	8	Switch	8.4 ± 3.6	<1	100	100	1.3 ± 0.3	<1
CD34⁺38^low19⁻10⁻	4	Lymphoid	30 ± 11	0	100	100	6.3 ± 3	0

One thousand sorted cells of the indicated phenotype were cultured in 24 wells precoated with confluent MS-5 cells in either lymphoid or switch conditions (see the Materials and Methods for details). At the end of the 6-week culture period, cell phenotype was analyzed by flow cytometry. The proportion of CD19⁺ cells was calculated in a morphologic gate including 80% of nucleated cells. The proportion of CD19⁺ cells coexpressing another B-lineage–specific marker was calculated in each experiment and the mean ± SEM of these calculations is shown.

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