Comparison Between the Observed and Expected Amounts of Hemoglobin Tetramers in Transgenic Sickle Cell Mice
| Mice . | Type of Tetramer . | Amount Observed . | Amount Expected . | Ratio of Observed/Expected . |
|---|---|---|---|---|
| M-Tg58Ru | mα2mβ2 | 51.89 | 46.39 | 1.12 |
| mα2hβSAnt2 | 18.97 | 24.85 | 0.76 | |
| hα2mβ2 | 13.48 | 18.82 | 0.72 | |
| hα2hβSAnt2 | 15.69 | 10.15 | 1.55 | |
| M-Tg98Ru | mα2mβ2 | 49.63 | 45.75 | 1.08 |
| mα2hβSAnt2 | 12.15 | 15.25 | 0.80 | |
| hα2mβ2 | 25.03 | 29.25 | 0.86 | |
| hα2hβSAnt2 | 13.36 | 9.75 | 1.37 | |
| Tg58 × Tg98 | mα2mβ2 | 32.46 | 27.03 | 1.20 |
| mα2hβSAnt2 | 14.53 | 24.48 | 0.59 | |
| hα2mβ2 | 15.09 | 25.97 | 0.58 | |
| hα2hβSAnt2 | 37.94 | 23.52 | 1.61 |
| Mice . | Type of Tetramer . | Amount Observed . | Amount Expected . | Ratio of Observed/Expected . |
|---|---|---|---|---|
| M-Tg58Ru | mα2mβ2 | 51.89 | 46.39 | 1.12 |
| mα2hβSAnt2 | 18.97 | 24.85 | 0.76 | |
| hα2mβ2 | 13.48 | 18.82 | 0.72 | |
| hα2hβSAnt2 | 15.69 | 10.15 | 1.55 | |
| M-Tg98Ru | mα2mβ2 | 49.63 | 45.75 | 1.08 |
| mα2hβSAnt2 | 12.15 | 15.25 | 0.80 | |
| hα2mβ2 | 25.03 | 29.25 | 0.86 | |
| hα2hβSAnt2 | 13.36 | 9.75 | 1.37 | |
| Tg58 × Tg98 | mα2mβ2 | 32.46 | 27.03 | 1.20 |
| mα2hβSAnt2 | 14.53 | 24.48 | 0.59 | |
| hα2mβ2 | 15.09 | 25.97 | 0.58 | |
| hα2hβSAnt2 | 37.94 | 23.52 | 1.61 |
The amount of Hb tetramers expected if their assembly was proportional to the product of the concentration of the globins in the RBCs.