Effect of IL-10, IFN-γ, and Hydrocortisone on Survival and Apoptosis in B-CLL Cells
| UPN . | Medium . | Experimental Culture Condition . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Medium + IL-10 . | Medium + IFN-γ . | Medium + HC . | Medium + HC + IL-10 . | Medium + HC + IFN-γ . | . | . | . | |||||
| . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| % V | % A | % V | % A | % V | % A | % V | % A | % V | % A | % V | % A | ||||
| 1 | 90.2 | 32.6 | 92.6 | 13.7 | 96.2 | 8.8 | 63.7 | 67.6 | 69.8 | 57.5 | 76.3 | 44.5 | |||
| 2 | 90.6 | 8.2 | 96.3 | 6.6 | 96.5 | 4.5 | 79.7 | 27.6 | 89.8 | 16.8 | 88.5 | 16.8 | |||
| 3 | 94.6 | 14.5 | 90.3 | 13.0 | 95.6 | 7.4 | 65.3 | 45.8 | 77.1 | 37.4 | 78.9 | 29.7 | |||
| 4 | 83.7 | 27.8 | 95.1 | 10.2 | 96.9 | 6.7 | 74.0 | 37.6 | 93.3 | 13.2 | 91.1 | 15.0 | |||
| 5 | 90.7 | 18.2 | 93.5 | 13.5 | 97.3 | 10.3 | 76.5 | 37.6 | 82.4 | 25.1 | 83.4 | 22.2 | |||
| 6 | 88.0 | 17.1 | 94.3 | 13.8 | 94.3 | 11.7 | 59.8 | 67.9 | 71.3 | 53.9 | 76.5 | 52.9 | |||
| 7 | 96.9 | 6.7 | 96.9 | 5.7 | 97.1 | 5.6 | 90.1 | 19.2 | 92.9 | 13.8 | 90.6 | 14.4 | |||
| Mean | 90.7 | 17.9 | 94.1 | 10.9 | 96.3 | 7.9 | 72.7 | 43.3 | 82.4 | 31.1 | 83.6 | 27.9 | |||
| UPN . | Medium . | Experimental Culture Condition . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Medium + IL-10 . | Medium + IFN-γ . | Medium + HC . | Medium + HC + IL-10 . | Medium + HC + IFN-γ . | . | . | . | |||||
| . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| % V | % A | % V | % A | % V | % A | % V | % A | % V | % A | % V | % A | ||||
| 1 | 90.2 | 32.6 | 92.6 | 13.7 | 96.2 | 8.8 | 63.7 | 67.6 | 69.8 | 57.5 | 76.3 | 44.5 | |||
| 2 | 90.6 | 8.2 | 96.3 | 6.6 | 96.5 | 4.5 | 79.7 | 27.6 | 89.8 | 16.8 | 88.5 | 16.8 | |||
| 3 | 94.6 | 14.5 | 90.3 | 13.0 | 95.6 | 7.4 | 65.3 | 45.8 | 77.1 | 37.4 | 78.9 | 29.7 | |||
| 4 | 83.7 | 27.8 | 95.1 | 10.2 | 96.9 | 6.7 | 74.0 | 37.6 | 93.3 | 13.2 | 91.1 | 15.0 | |||
| 5 | 90.7 | 18.2 | 93.5 | 13.5 | 97.3 | 10.3 | 76.5 | 37.6 | 82.4 | 25.1 | 83.4 | 22.2 | |||
| 6 | 88.0 | 17.1 | 94.3 | 13.8 | 94.3 | 11.7 | 59.8 | 67.9 | 71.3 | 53.9 | 76.5 | 52.9 | |||
| 7 | 96.9 | 6.7 | 96.9 | 5.7 | 97.1 | 5.6 | 90.1 | 19.2 | 92.9 | 13.8 | 90.6 | 14.4 | |||
| Mean | 90.7 | 17.9 | 94.1 | 10.9 | 96.3 | 7.9 | 72.7 | 43.3 | 82.4 | 31.1 | 83.6 | 27.9 | |||
Abbreviations: UPN, unique patient number; IL-10, interleukin-10 (25 ng/mL); IFN-γ, interferon gamma (100 U/mL); HC, hydrocortisone (5 × 104 mol/L); % V, percent viability as determined by PI exclusion and flow cytometry; % A, percent apoptosis as determined by PI nuclear staining and flow cytometry.