Strategy for Detection of L-PK Gene Mutations
| . | Primers . | PCR Products (bp) . | Restriction Enzyme . | Normal Allele . | Mutant Allele . |
|---|---|---|---|---|---|
| Ivs7[+1]gt→tt | R-6/R-7 | 351 | Hph I | 178 + 88 + 58 + 27 | 205 + 88 + 58 |
| 1269GCG→GCA | R-8/R-9 | 170 | Pst I | 115 + 55 | 95 + 55 + 20 |
| Ivs 3[−2]ag→tg | R-10/R-11 | 179 | Bfa I | 95 + 84 | 179 |
| 1044AAG→AAT | R-12/R-13 | 83 | Hph I | 60 + 14 + 9 | 69 + 14 |
| 1075CGC→TGC | R-12/R-14 | 134 | Hha I | 94 + 40 | 134 |
| . | Primers . | PCR Products (bp) . | Restriction Enzyme . | Normal Allele . | Mutant Allele . |
|---|---|---|---|---|---|
| Ivs7[+1]gt→tt | R-6/R-7 | 351 | Hph I | 178 + 88 + 58 + 27 | 205 + 88 + 58 |
| 1269GCG→GCA | R-8/R-9 | 170 | Pst I | 115 + 55 | 95 + 55 + 20 |
| Ivs 3[−2]ag→tg | R-10/R-11 | 179 | Bfa I | 95 + 84 | 179 |
| 1044AAG→AAT | R-12/R-13 | 83 | Hph I | 60 + 14 + 9 | 69 + 14 |
| 1075CGC→TGC | R-12/R-14 | 134 | Hha I | 94 + 40 | 134 |