Effect of IL-3 and SCF on Hemoglobin Synthesis in UT-7/GM Cells
| Cytokines . | Dianisidine-Positive Cells (%) . |
|---|---|
| EPO | 93.0 ± 1.0 |
| TPO | 7.5 ± 0.6 |
| GM-CSF | 0 |
| GM-CSF + EPO | 0 |
| GM-CSF + TPO | 0 |
| GM-CSF + IL-3 | 0 |
| GM-CSF + SCF | 0 |
| IL-3 | 7.7 ± 0.6 |
| IL-3 + EPO | 24.3 ± 1.5 |
| IL-3 + TPO | 5.7 ± 0.6 |
| SCF | 11.0 ± 1.0 |
| SCF + EPO | 46.0 ± 1.7 |
| SCF + TPO | 9.0 ± 2.7 |
| Cytokines . | Dianisidine-Positive Cells (%) . |
|---|---|
| EPO | 93.0 ± 1.0 |
| TPO | 7.5 ± 0.6 |
| GM-CSF | 0 |
| GM-CSF + EPO | 0 |
| GM-CSF + TPO | 0 |
| GM-CSF + IL-3 | 0 |
| GM-CSF + SCF | 0 |
| IL-3 | 7.7 ± 0.6 |
| IL-3 + EPO | 24.3 ± 1.5 |
| IL-3 + TPO | 5.7 ± 0.6 |
| SCF | 11.0 ± 1.0 |
| SCF + EPO | 46.0 ± 1.7 |
| SCF + TPO | 9.0 ± 2.7 |
UT-7/GM cells were cultured in tissue culture flasks with EPO (10 U/mL), TPO (100 ng/mL), GM-CSF (10 ng/mL), IL-3 (10 ng/mL), SCF (100 ng/mL), or their combinations. Two weeks later, the cells were harvested and prepared for dianidisine staining. Dianidisine-positive cells were counted. The data are mean ± SD from triplicate cultures.