Colony Formation by Lin− Sca-1+ Fractions
| Fractions . | No. of Colonies per 100 Sorted Cells* . |
|---|---|
| c-Kit + | 69.3 ± 2.9 |
| c-Kit + TIE− | 62.0 ± 2.2 |
| c-Kit + TIE+ | 68.9 ± 2.0 |
| c-Kit + TEK− | 62.7 ± 0.9 |
| c-Kit + TEK+ | 69.3 ± 2.4 |
| Fractions . | No. of Colonies per 100 Sorted Cells* . |
|---|---|
| c-Kit + | 69.3 ± 2.9 |
| c-Kit + TIE− | 62.0 ± 2.2 |
| c-Kit + TIE+ | 68.9 ± 2.0 |
| c-Kit + TEK− | 62.7 ± 0.9 |
| c-Kit + TEK+ | 69.3 ± 2.4 |
For the progenitor assay, 100 sorted cells were embedded in 1 mL of methylcellulose medium containing SCF, IL-3, IL-6, and Epo. The numbers of colonies were scored on day 10 of culture.
Mean ± SD for triplicates.