Neutrophil Respiratory Burst Activity, Adhesion Receptor Expression and Fas Expression in Response to Activating Factors
| . | Control . | LPS (1 μg/mL) . | Migrated . | Cross-linked . |
|---|---|---|---|---|
| Reactive oxygen intermediates (LnMCF) | 14.4 ± 12.0 | 34.9 ± 3.6* | 22.1 ± 3.6* | 25.6 ± 4.0* |
| CD11b (LnMCF) | 88.3 ± 9.4 | 144.3 ± 11.5* | 120.0 ± 7.3* | — |
| L-selectin (LnMCF) | 12.3 ± 2.7 | 3.9 ± 0.6* | 6.1 ± 2.0* | — |
| Fas (LnMCF) | 8.4 ± 3.9 | 8.8 ± 2.6 | 9.1 ± 3.1 | 7.9 ± 1.8 |
| . | Control . | LPS (1 μg/mL) . | Migrated . | Cross-linked . |
|---|---|---|---|---|
| Reactive oxygen intermediates (LnMCF) | 14.4 ± 12.0 | 34.9 ± 3.6* | 22.1 ± 3.6* | 25.6 ± 4.0* |
| CD11b (LnMCF) | 88.3 ± 9.4 | 144.3 ± 11.5* | 120.0 ± 7.3* | — |
| L-selectin (LnMCF) | 12.3 ± 2.7 | 3.9 ± 0.6* | 6.1 ± 2.0* | — |
| Fas (LnMCF) | 8.4 ± 3.9 | 8.8 ± 2.6 | 9.1 ± 3.1 | 7.9 ± 1.8 |
Neutrophils were incubated with LPS (1 μg/mL), migrated or cross-linked with anti-CD11b and then assessed for reactive oxygen intermediates 5, 10, and 8 minutes, respectively. Surface receptor expression of CD11b and L-selectin were assessed in LPS and migrated neutrophils. Results are the mean ± SD of n = 3 experiments.
P < .05 (not performed because of cross-linking interface to antibodies).