AChE Activity in Human Megakaryoblastic Cell Lines
| Cell Line . | mOD/min . | mOD/min + BW . | mOD/min + IO . | nmol/min/cell . | nmol/min/cell . | Calc. No. of . |
|---|---|---|---|---|---|---|
| . | None . | . | . | BChE . | AChE . | Molecules/Cell . |
| DAMI | 3.630 | 0.607 | 3.589 | 0.67 × 10−7 | 5.05 × 10−7 | 84 |
| MEG01 | 1.529 | 0.300 | 0.925 | 0.22 × 10−7 | 1.14 × 10−7 | 19 |
| CHRF | 0.932 | 0.320 | 0.804 | 0.26 × 10−7 | 0.96 × 10−7 | 16 |
| Cell Line . | mOD/min . | mOD/min + BW . | mOD/min + IO . | nmol/min/cell . | nmol/min/cell . | Calc. No. of . |
|---|---|---|---|---|---|---|
| . | None . | . | . | BChE . | AChE . | Molecules/Cell . |
| DAMI | 3.630 | 0.607 | 3.589 | 0.67 × 10−7 | 5.05 × 10−7 | 84 |
| MEG01 | 1.529 | 0.300 | 0.925 | 0.22 × 10−7 | 1.14 × 10−7 | 19 |
| CHRF | 0.932 | 0.320 | 0.804 | 0.26 × 10−7 | 0.96 × 10−7 | 16 |
Cell extracts were prepared in high salt detergent buffer and AChE activities in total cell homogenates were determined as detailed in Materials and Methods. Raw data are presented as mOD/min of 1 mmol/L acetylthiocholine hydrolyzed24 in the presence of the AChE inhibitor BW284C51 (BW) at 1 × 10−5 mol/L, the BuChE inhibitor iso-OMPA (IO) at 1 × 10−4 mol/L, or without inhibitors (none). Linearity of the reaction24 was stable throughout detection (correlation coefficient > 0.99). Because of the low-leveled activity values, spontaneous substrate hydrolysis in extraction buffer (0.151 mOD/min) was subtracted and net activity divided by cell numbers.