Correlation Between SAg-Induced T-Cell Activation and PMN Class II Expression
| Experiment . | Donor . | MHC Class II Expression (MFI)* . | [3H]TdR Incorporation cPM)† . | ||
|---|---|---|---|---|---|
| . | . | Control . | GM-CSF/IFN-γ . | Control . | GM-CSF/IFN-γ . |
| 1 | 1 | 14 ± 0 | 278 ± 7 | 1,813 ± 764 | 10,986 ± 515 |
| 8 | 9 ± 1 | 34 ± 1 | 941 ± 253 | 1,980 ± 344 | |
| 2 | 4 | 18 ± 1 | 185 ± 4 | 823 ± 305 | 21,863 ± 5,378 |
| 9 | 17 ± 1 | 28 ± 2 | 458 ± 250 | 6,663 ± 847 | |
| 3 | 5 | 10 ± 1 | 149 ± 3 | 1,220 ± 489 | 14,367 ± 3,452 |
| 10 | 10 ± 0 | 32 ± 1 | 730 ± 104 | 4,101 ± 1,283 | |
| 4 | 2 | 18 ± 0 | 180 ± 3 | 2,283 ± 814 | 27,760 ± 3,364 |
| 7 | 13 ± 0 | 55 ± 2 | 1,156 ± 234 | 5,377 ± 1,155 | |
| Experiment . | Donor . | MHC Class II Expression (MFI)* . | [3H]TdR Incorporation cPM)† . | ||
|---|---|---|---|---|---|
| . | . | Control . | GM-CSF/IFN-γ . | Control . | GM-CSF/IFN-γ . |
| 1 | 1 | 14 ± 0 | 278 ± 7 | 1,813 ± 764 | 10,986 ± 515 |
| 8 | 9 ± 1 | 34 ± 1 | 941 ± 253 | 1,980 ± 344 | |
| 2 | 4 | 18 ± 1 | 185 ± 4 | 823 ± 305 | 21,863 ± 5,378 |
| 9 | 17 ± 1 | 28 ± 2 | 458 ± 250 | 6,663 ± 847 | |
| 3 | 5 | 10 ± 1 | 149 ± 3 | 1,220 ± 489 | 14,367 ± 3,452 |
| 10 | 10 ± 0 | 32 ± 1 | 730 ± 104 | 4,101 ± 1,283 | |
| 4 | 2 | 18 ± 0 | 180 ± 3 | 2,283 ± 814 | 27,760 ± 3,364 |
| 7 | 13 ± 0 | 55 ± 2 | 1,156 ± 234 | 5,377 ± 1,155 | |
PMN SAg-induced T-cell proliferation is presented for four separate experiments, each using one HLA-DRhigh and HLA-DRlow responding pair. As described in Materials and Methods, 1 × 105 PMN cultured in the presence or absence of 100 U/mL of GM-CSF/IFN-γ for 44 hours were incubated with 5 × 104 CD4+ T lymphocytes and 10 nmol/L SEA.
The level of class II induction for both the unstimulated and GM-CSF/IFN-γ–stimulated PMN was determined by FACScan analysis and is presented as the MFI.
PMN SAg-induced T-cell proliferation is presented as counts per minute (cpm). Values reflect the amount of [3H]TdR incorporated following an 18 to 20-hour pulse and are the mean of triplicate samples ± SD.