Evidence for An Accessory Cell Interaction
| No. Donor Rh/Hoedull Cells . | No. of Starting Whole Marrow Cells (×106) . | % Male Expected From Equivalent Whole Marrow Cells4-150 . | Actual % Male in Whole Marrow From Rh/Hoedull Cells Using Southern Blot Analysis . | % of Expected Value . |
|---|---|---|---|---|
| 2,600 | 87 | 22 | 1.4 | 6.4 |
| 3,000 | 100 | 25 | 0.9 | 3.6 |
| 5,500 | 183 | 46 | 1.2 | 3.3 |
| 10,000 | 333 | 83 | 0.7 | 0.8 |
| 10,000 | 333 | 83 | 2.2 | 2.6 |
| No. Donor Rh/Hoedull Cells . | No. of Starting Whole Marrow Cells (×106) . | % Male Expected From Equivalent Whole Marrow Cells4-150 . | Actual % Male in Whole Marrow From Rh/Hoedull Cells Using Southern Blot Analysis . | % of Expected Value . |
|---|---|---|---|---|
| 2,600 | 87 | 22 | 1.4 | 6.4 |
| 3,000 | 100 | 25 | 0.9 | 3.6 |
| 5,500 | 183 | 46 | 1.2 | 3.3 |
| 10,000 | 333 | 83 | 0.7 | 0.8 |
| 10,000 | 333 | 83 | 2.2 | 2.6 |
The expected values for 100 × 106 whole marrow cells was determined from Southern blot analysis of at least 3 previous experiments giving these equivalent numbers of whole marrow cells. The expected values for the other whole marrow cell doses were theoretically calculated based on a linear relationship between the number of cells given and the % donor cells detected at 6 weeks to 6 months.