Transduction Efficiency (Median Percent and Range) of Primary Leukemia Cells
| . | Vector Type . | ||
|---|---|---|---|
| . | Retroviral* . | Adenoviral† . | HSV-DISC‡ . |
| ALLρ (n = 5) | 1 (0-3) | 0 (0) | 100 (85-100) |
| AML (n = 5) | 6 (2-12) | 10 (0-35) | 86 (80-98) |
| . | Vector Type . | ||
|---|---|---|---|
| . | Retroviral* . | Adenoviral† . | HSV-DISC‡ . |
| ALLρ (n = 5) | 1 (0-3) | 0 (0) | 100 (85-100) |
| AML (n = 5) | 6 (2-12) | 10 (0-35) | 86 (80-98) |
Retroviral vector used was G1-NGFR, derived from the Moloney virus backbone, and encoding a truncated nerve growth factor receptor. Percent positivity was determined by FACS analysis.38 MOI used were 5 and 10/cell.
Adenoviral vector used was an E1/E3 deletion mutant containing the lac-Z/β-Gal marker. MOI used were 30 and 100/cell. Percent positivity was determined by FACS analysis.
HSV-DISC β-Gal vector used as described in Materials and Methods. MOI used was 0.05 pfu/cell.
ρ Results show the maximum transduction efficiency obtained for each of the patients studied.