Two-Step In Vitro Differentiation of Wild-Type and GATA-2−/− ES Cells (colonies/105 cells in secondary plates)
| Genotype . | Eryp . | Eryd . | Mac . | Non-Red/G . | Mix/Blast . | Total . |
|---|---|---|---|---|---|---|
| Wild-type | ||||||
| No. | 106 ± 31 | 62 ± 14 | 220 ± 11 | 78 ± 14 | 80 ± 11 | 546 ± 3 |
| % | 19.4 | 11.4 | 40.3 | 14.3 | 14.6 | 100 |
| GATA-2−/− | ||||||
| No. | 20 ± 3 | 15 ± 1 | 86 ± 6 | 4 ± 1 | 2 ± 1 | 127 ± 4 |
| % | 15.7 | 11.8 | 67.7 | 3.2 | 1.6 | 100 |
| Genotype . | Eryp . | Eryd . | Mac . | Non-Red/G . | Mix/Blast . | Total . |
|---|---|---|---|---|---|---|
| Wild-type | ||||||
| No. | 106 ± 31 | 62 ± 14 | 220 ± 11 | 78 ± 14 | 80 ± 11 | 546 ± 3 |
| % | 19.4 | 11.4 | 40.3 | 14.3 | 14.6 | 100 |
| GATA-2−/− | ||||||
| No. | 20 ± 3 | 15 ± 1 | 86 ± 6 | 4 ± 1 | 2 ± 1 | 127 ± 4 |
| % | 15.7 | 11.8 | 67.7 | 3.2 | 1.6 | 100 |
Results (mean ± SD) are from 2 experiments with 2 independent GATA-2−/− ES clones.
Abbreviations: Eryp, primitive erythroid colonies; Eryd, definitive erythroid colonies; Mac, macrophage colonies; Non-Red/G, non-red colonies with granulated cells (mostly mast cell colonies at this stage); Mix/Blast, mixed or blast cell colonies.