Table 1.

Limiting Dilution Analysis of Human CB, Adult BM, and Mobilized PB

Cell SourceCell DoseNo. of Negative MiceNo. of Transplanted MicePercentage of Mice Negative
CB (n = 7) 1 × 105 11 11 100 
 5 × 105 8 12 67 
 1 × 106 10 37 27 
 4 × 106 2 24 8 
 8 × 106 0 7 0 
BM (n = 5) 5 × 105 6 9 67 
 1 × 106 18 25 72 
 2 × 106 6 9 67 
 4 × 106 4 19 21 
 8 × 106 1 5 20 
Mobilized PB (n = 7) 2.5 × 106 3 8 38 
 5 × 106 17 32 53 
 10 × 106 2 14 14 
 20 × 106 2 16 13 
Cell SourceCell DoseNo. of Negative MiceNo. of Transplanted MicePercentage of Mice Negative
CB (n = 7) 1 × 105 11 11 100 
 5 × 105 8 12 67 
 1 × 106 10 37 27 
 4 × 106 2 24 8 
 8 × 106 0 7 0 
BM (n = 5) 5 × 105 6 9 67 
 1 × 106 18 25 72 
 2 × 106 6 9 67 
 4 × 106 4 19 21 
 8 × 106 1 5 20 
Mobilized PB (n = 7) 2.5 × 106 3 8 38 
 5 × 106 17 32 53 
 10 × 106 2 14 14 
 20 × 106 2 16 13 

NOD/SCID mice were transplanted with serial doses of mononuclear cells from human CB (n mice = 91), BM (n = 67), or G-CSF–mobilized PB (n = 70) and the murine BM was analyzed after 4 to 6 weeks by Southern blot to determine the level of human cell engraftment. Mice were scored as negative if no human cells were detectable. The number of experiments from which the pooled data are taken is indicated for each cell source in parentheses.

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