Effects of PEG-rHuMGDF on Marrow Megakaryocytes and Platelet Production in Normal Rhesus Macaques
| Duration PEG-rHu MGDF Therapy (2.5 μg/kg/d) . | Megakaryocytes . | Platelets . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | Ratio to NE (×103)* . | No. . | Volume (×103 fL) . | Mass . | Increase (×N) . | Concentration (×109/L) . | Volume (fL) . | Mass turnover . | Increase (×N) . |
| . | . | (×106 megas/kg) . | . | (×1010 fL/kg) . | . | . | . | (×105 fL plats/μL/d) . | . |
| Baseline | 1.20 ± 0.26 | 6.36 ± 1.41 | 30.1 ± 3.60 | 19.5 ± 6.62 | 1.0 | 416 ± 88 | 8.3 ± 1.0 | 5.17 ± 1.27 | 1.0 |
| 3 d | 2.21 ± 0.84 | 11.7 ± 4.45 | 57.2 ± 3.39 | 67.4 ± 29.5 | 3.4 | 474 ± 108 | 8.0 ± 0.5 | 7.95 ± 1.64 | 1.5 |
| 7 d | 6.25 ± 2.73 | 33.2 ± 14.6 | 48.6 ± 1.44 | 160 ± 68.6 | 8.2 | 1,084 ± 153 | 7.6 ± 0.9 | 17.2 ± 2.49 | 3.2 |
| 14 d | 8.42 ± 3.52 | 55.5 ± 11.7 | 40.1 ± 8.71 | 231 ± 7.20 | 11.8 | 2,197 ± 249 | 7.2 ± 0.9 | 32.7 ± 1.52 | 6.2 |
| Duration PEG-rHu MGDF Therapy (2.5 μg/kg/d) . | Megakaryocytes . | Platelets . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | Ratio to NE (×103)* . | No. . | Volume (×103 fL) . | Mass . | Increase (×N) . | Concentration (×109/L) . | Volume (fL) . | Mass turnover . | Increase (×N) . |
| . | . | (×106 megas/kg) . | . | (×1010 fL/kg) . | . | . | . | (×105 fL plats/μL/d) . | . |
| Baseline | 1.20 ± 0.26 | 6.36 ± 1.41 | 30.1 ± 3.60 | 19.5 ± 6.62 | 1.0 | 416 ± 88 | 8.3 ± 1.0 | 5.17 ± 1.27 | 1.0 |
| 3 d | 2.21 ± 0.84 | 11.7 ± 4.45 | 57.2 ± 3.39 | 67.4 ± 29.5 | 3.4 | 474 ± 108 | 8.0 ± 0.5 | 7.95 ± 1.64 | 1.5 |
| 7 d | 6.25 ± 2.73 | 33.2 ± 14.6 | 48.6 ± 1.44 | 160 ± 68.6 | 8.2 | 1,084 ± 153 | 7.6 ± 0.9 | 17.2 ± 2.49 | 3.2 |
| 14 d | 8.42 ± 3.52 | 55.5 ± 11.7 | 40.1 ± 8.71 | 231 ± 7.20 | 11.8 | 2,197 ± 249 | 7.2 ± 0.9 | 32.7 ± 1.52 | 6.2 |
Ratio of marrow megakaryocytes to nucleated erythroid cells determined by comparing nucleated GPIIb/IIIa-positive cells to nucleated glycophorin A-positive cells using flow cytometry (see Materials and Methods).