IL-6 Production by Macrophage Generated From Monocytes Cultured With M-CSF or M-CSF + IL-10
| Stimulated With . | IL-6 (ng/105 cells) . | |
|---|---|---|
| . | M-CSF . | M-CSF + IL-10 . |
| — | 0.2 ± 0.3 | 0.3 ± 0.4 |
| LPS | 13.0 ± 6.9 | 39.0 ± 11.55-150 |
| LPS + IL-10 | 3.2 ± 0.5 | 8.1 ± 1.4 |
| Stimulated With . | IL-6 (ng/105 cells) . | |
|---|---|---|
| . | M-CSF . | M-CSF + IL-10 . |
| — | 0.2 ± 0.3 | 0.3 ± 0.4 |
| LPS | 13.0 ± 6.9 | 39.0 ± 11.55-150 |
| LPS + IL-10 | 3.2 ± 0.5 | 8.1 ± 1.4 |
Monocyte were incubated with rhM-CSF (50 ng/mL) or rhM-CSF + IL-10 (25 ng/mL) for 1 week, then recovered macrophages were stimulated with LPS (100 ng/mL) in the presence or absence of IL-10 for 24 hours. IL-6 in the culture supernatant was measured using an ELISA.
P < .05 versus M-CSF alone (LPS stimulation).