FcγR-Mediated Phagocytosis Activity of Macrophages Generated From Monocytes Cultured With M-CSF or M-CSF + IL-10
| Mφ Recovered From Culture With . | IL-10 . | Phagocytosis of EA (%) . | |
|---|---|---|---|
| . | . | 20 min . | 60 min . |
| M-CSF | − | 64.8 ± 6.8 | 100 |
| + | 65.1 ± 4.7 | 100 | |
| M-CSF + IL-10 | − | 82.8 ± 8.24-150 | 100 |
| + | 84.1 ± 4.94-150 | 100 | |
| Mφ Recovered From Culture With . | IL-10 . | Phagocytosis of EA (%) . | |
|---|---|---|---|
| . | . | 20 min . | 60 min . |
| M-CSF | − | 64.8 ± 6.8 | 100 |
| + | 65.1 ± 4.7 | 100 | |
| M-CSF + IL-10 | − | 82.8 ± 8.24-150 | 100 |
| + | 84.1 ± 4.94-150 | 100 | |
Monocytes were incubated with rhM-CSF (50 ng/mL) plus rhIL-10 (25 ng/mL), or rhM-CSF alone for 1 week. The resulting macrophages were incubated with EA in the presence or absence of IL-10 for 20 or 60 minutes at 37°C. Data are expressed as the means ± SD of three experiments.
P < .05 versus M-CSF alone (20 minutes).