Zymosan Stimulated O2-Production in Monocytes-Derived Macrophage
| Mφ Recovered From Culture With . | IL-10 . | Peak Count (×106 CPM) . | ||
|---|---|---|---|---|
| . | . | Exp. 1 . | Exp. 2 . | Exp. 3 . |
| M-CSF | − | 16.4 | 15.1 | 12.0 |
| + | 15.4 | 15.5 | 12.2 | |
| M-CSF + IL-10 | − | 26.5 | 25.4 | 24.9 |
| + | 27.1 | 25.0 | 24.3 | |
| Mφ Recovered From Culture With . | IL-10 . | Peak Count (×106 CPM) . | ||
|---|---|---|---|---|
| . | . | Exp. 1 . | Exp. 2 . | Exp. 3 . |
| M-CSF | − | 16.4 | 15.1 | 12.0 |
| + | 15.4 | 15.5 | 12.2 | |
| M-CSF + IL-10 | − | 26.5 | 25.4 | 24.9 |
| + | 27.1 | 25.0 | 24.3 | |
Monocytes were incubated with M-CSF (50 ng/mL) + IL-10 (25 ng/mL) or M-CSF alone for 7 days. Recovered macrophages were washed, then the reaction was started by adding zymosan (1 mg/mL) in the presence or absence of IL-10. CL of these macrophages (5 × 105) was measured as described in Materials and Methods. Data are results from three independent experiments.