IL-10 Enhances the M-CSF–Dependent Growth of Human Monocytes
| IL-10 (ng/mL) . | Cell No. (×104) . | |
|---|---|---|
| . | − M-CSF . | + M-CSF . |
| 0 | 0.5 ± 0.2 | 9.3 ± 0.8 |
| 1 | 0.6 ± 0.3 | 10.8 ± 0.6 |
| 10 | 0.2 ± 0.2 | 12.1 ± 0.6* |
| 100 | 0.1 ± 0.2 | 12.4 ± 0.3* |
| IL-10 (ng/mL) . | Cell No. (×104) . | |
|---|---|---|
| . | − M-CSF . | + M-CSF . |
| 0 | 0.5 ± 0.2 | 9.3 ± 0.8 |
| 1 | 0.6 ± 0.3 | 10.8 ± 0.6 |
| 10 | 0.2 ± 0.2 | 12.1 ± 0.6* |
| 100 | 0.1 ± 0.2 | 12.4 ± 0.3* |
Monocytes (8.8 × 104) in 24-well tissue culture plates were incubated with IL-10 (1, 10, 100 ng/mL), M-CSF (50 ng/mL), M-CSF + IL-10 or medium alone for 7 days. The cells were counted using cetrimide as described in Materials and Methods. Data are expressed as the mean ± SD from three wells in triplicate experiments.
P < .05 versus IL-10, 0 ng/mL.