Distribution of Lymphocytes in Different Phases of the Cell Cycle as a Function of the Time of Stimulation Determined From Flow Cytometric and Microscopic Data
| Radiation Dose (Gy) . | Time of Stimulation (h) . | No. of Cells (%) . | |||
|---|---|---|---|---|---|
| . | . | G0 . | G1 . | S . | G2 . |
| 0 | 83.0 ± 5.4 | 14.0 ± 2.9 | 3.0 ± 2.9 | 0 ± 2.5 | |
| 0 | 20 | 56.0 ± 6.3 | 28.2 ± 2.8 | 14.5 ± 3.8 | 1.3 ± 2.5 |
| 30 | 17.0 ± 6.9 | 65.1 ± 4.4 | 13.2 ± 4.1 | 4.7 ± 2.8 | |
| 40 | 78.7 ± 6.3* | 15.8 ± 3.6 | 5.5 ± 2.7 | ||
| 50 | 25.0 ± 2.6 | 54.7 ± 4.2 | 10.6 ± 3.7 | 9.7 ± 3.1 | |
| 20 | 80.9 ± 6.2* | 12.5 ± 3.4 | 6.5 ± 2.8 | ||
| 5 | 30 | 30.0 ± 2.7 | 43.4 ± 3.0 | 17.6 ± 3.1 | 8.0 ± 2.6 |
| 40 | 76.2 ± 6.9* | 13.7 ± 3.8 | 10.1 ± 3.1 | ||
| 50 | 26.0 ± 2.5 | 51.7 ± 5.0 | 12.0 ± 4.1 | 10.3 ± 3.4 | |
| Radiation Dose (Gy) . | Time of Stimulation (h) . | No. of Cells (%) . | |||
|---|---|---|---|---|---|
| . | . | G0 . | G1 . | S . | G2 . |
| 0 | 83.0 ± 5.4 | 14.0 ± 2.9 | 3.0 ± 2.9 | 0 ± 2.5 | |
| 0 | 20 | 56.0 ± 6.3 | 28.2 ± 2.8 | 14.5 ± 3.8 | 1.3 ± 2.5 |
| 30 | 17.0 ± 6.9 | 65.1 ± 4.4 | 13.2 ± 4.1 | 4.7 ± 2.8 | |
| 40 | 78.7 ± 6.3* | 15.8 ± 3.6 | 5.5 ± 2.7 | ||
| 50 | 25.0 ± 2.6 | 54.7 ± 4.2 | 10.6 ± 3.7 | 9.7 ± 3.1 | |
| 20 | 80.9 ± 6.2* | 12.5 ± 3.4 | 6.5 ± 2.8 | ||
| 5 | 30 | 30.0 ± 2.7 | 43.4 ± 3.0 | 17.6 ± 3.1 | 8.0 ± 2.6 |
| 40 | 76.2 ± 6.9* | 13.7 ± 3.8 | 10.1 ± 3.1 | ||
| 50 | 26.0 ± 2.5 | 51.7 ± 5.0 | 12.0 ± 4.1 | 10.3 ± 3.4 | |
The cell cycle phases were determined by flow cytometry (G0 + G1 , S, and G2 ) and according to the nuclear radius (R)31 (R < 5 μm for G0 , R > 5 μm for G1 + S + G2 ).
Microscopic analysis was not performed.