Cytospin Preparations (Wright-Giemsa), Flow Cytometry, and Plating Efficiency of Nonmigrating and Transmigrated Cells in Continuous Coculture With Immortalized BMEC
. | Day 15 . | Day 30 . | ||
---|---|---|---|---|
. | Nonmigrating Cells . | Transmigrated Cells . | Nonmigrating Cells . | Transmigrated Cells . |
Myeloblasts (%) | 3.8 ± 1.8 | 2.3 ± 1.4 | 0 | 0 |
Myeloid Precursors (%) | 78.3 ± 2.5 | 3.8 ± 1.1 | 5.3 ± 1.2 | 2.3 ± 0.7 |
Monocytes (%) | 13.5 ± 2.3 | 76.5 ± 7.4 | 26.0 ± 2.0 | 24.3 ± 5.4 |
Granulocytes + bands (%) | 4.5 ± 1.2 | 17.5 ± 8.2 | 68.7 ± 1.5 | 73.3 ± 5.7 |
CD34+ (%) | 0 | 0 | 0 | 0 |
CD33+ (%) | 92.5 ± 3.5 | 94.6 ± 2.4 | 83.3 ± 6.6 | 91.0 ± 7.0 |
CD14++ (%) | 4.7 ± 2.3 | 49.1 ± 17.3 | 12.6 ± 4.2 | 28.5 ± 5.9 |
CD15++ (%) | 77.0 ± 7.6 | 23.0 ± 10.2 | 74.3 ± 4.0 | 64.1 ± 8.4 |
CD41a+ (%) | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.2 ± 0.1 | 0.8 ± 0.4 |
Plating efficiency (/10,000 cells) | 1.71 ± 0.37 | 1.33 ± 0.53 | 0.37 ± 0.03 | 0 |
. | Day 15 . | Day 30 . | ||
---|---|---|---|---|
. | Nonmigrating Cells . | Transmigrated Cells . | Nonmigrating Cells . | Transmigrated Cells . |
Myeloblasts (%) | 3.8 ± 1.8 | 2.3 ± 1.4 | 0 | 0 |
Myeloid Precursors (%) | 78.3 ± 2.5 | 3.8 ± 1.1 | 5.3 ± 1.2 | 2.3 ± 0.7 |
Monocytes (%) | 13.5 ± 2.3 | 76.5 ± 7.4 | 26.0 ± 2.0 | 24.3 ± 5.4 |
Granulocytes + bands (%) | 4.5 ± 1.2 | 17.5 ± 8.2 | 68.7 ± 1.5 | 73.3 ± 5.7 |
CD34+ (%) | 0 | 0 | 0 | 0 |
CD33+ (%) | 92.5 ± 3.5 | 94.6 ± 2.4 | 83.3 ± 6.6 | 91.0 ± 7.0 |
CD14++ (%) | 4.7 ± 2.3 | 49.1 ± 17.3 | 12.6 ± 4.2 | 28.5 ± 5.9 |
CD15++ (%) | 77.0 ± 7.6 | 23.0 ± 10.2 | 74.3 ± 4.0 | 64.1 ± 8.4 |
CD41a+ (%) | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.2 ± 0.1 | 0.8 ± 0.4 |
Plating efficiency (/10,000 cells) | 1.71 ± 0.37 | 1.33 ± 0.53 | 0.37 ± 0.03 | 0 |