Table 3.

Cytospin Preparations (Wright-Giemsa), Flow Cytometry, and Plating Efficiency of Nonmigrating and Transmigrated Cells in Continuous Coculture With Immortalized BMEC

Day 15Day 30
Nonmigrating CellsTransmigrated CellsNonmigrating CellsTransmigrated Cells
Myeloblasts (%) 3.8 ± 1.8 2.3 ± 1.4 
Myeloid Precursors (%) 78.3 ± 2.5 3.8 ± 1.1 5.3 ± 1.2 2.3 ± 0.7 
Monocytes (%) 13.5 ± 2.3 76.5 ± 7.4 26.0 ± 2.0 24.3 ± 5.4 
Granulocytes + bands (%) 4.5 ± 1.2 17.5 ± 8.2 68.7 ± 1.5 73.3 ± 5.7 
CD34+ (%) 
CD33+ (%) 92.5 ± 3.5 94.6 ± 2.4 83.3 ± 6.6 91.0 ± 7.0 
CD14++ (%) 4.7 ± 2.3 49.1 ± 17.3 12.6 ± 4.2 28.5 ± 5.9 
CD15++ (%) 77.0 ± 7.6 23.0 ± 10.2 74.3 ± 4.0 64.1 ± 8.4 
CD41a+ (%) 0.2 ± 0.2 0.2 ± 0.2 0.2 ± 0.1 0.8 ± 0.4 
Plating efficiency (/10,000 cells) 1.71 ± 0.37 1.33 ± 0.53 0.37 ± 0.03 
Day 15Day 30
Nonmigrating CellsTransmigrated CellsNonmigrating CellsTransmigrated Cells
Myeloblasts (%) 3.8 ± 1.8 2.3 ± 1.4 
Myeloid Precursors (%) 78.3 ± 2.5 3.8 ± 1.1 5.3 ± 1.2 2.3 ± 0.7 
Monocytes (%) 13.5 ± 2.3 76.5 ± 7.4 26.0 ± 2.0 24.3 ± 5.4 
Granulocytes + bands (%) 4.5 ± 1.2 17.5 ± 8.2 68.7 ± 1.5 73.3 ± 5.7 
CD34+ (%) 
CD33+ (%) 92.5 ± 3.5 94.6 ± 2.4 83.3 ± 6.6 91.0 ± 7.0 
CD14++ (%) 4.7 ± 2.3 49.1 ± 17.3 12.6 ± 4.2 28.5 ± 5.9 
CD15++ (%) 77.0 ± 7.6 23.0 ± 10.2 74.3 ± 4.0 64.1 ± 8.4 
CD41a+ (%) 0.2 ± 0.2 0.2 ± 0.2 0.2 ± 0.1 0.8 ± 0.4 
Plating efficiency (/10,000 cells) 1.71 ± 0.37 1.33 ± 0.53 0.37 ± 0.03 
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