Flow Cytometry of Nonmigrating and Transmigrated CD34+ Progenitor Cells
| Antibody . | Fluorescence Intensity (arbitrary units) . | |
|---|---|---|
| . | Nonmigrating Cells . | Transmigrated Cells . |
| IgG control (FITC) | 0.18 ± 0.01 | 0.20 ± 0.02 |
| IgG control (PE) | 0.21 ± 0.01 | 0.23 ± 0.01 |
| CD34 (PE) | 12.7 ± 3.8 | 14.1 ± 5.0 |
| CD38 (FITC) | 6.6 ± 0.8 | 10.0 ± 1.0* |
| L-selectin (FITC) | 4.1 ± 0.7 | 0.8 ± 0.1* |
| VLA-4 (FITC) | 2.3 ± 0.3 | 2.9 ± 0.5 |
| LFA-1 (PE) | 1.0 ± 0.4 | 1.1 ± 0.3 |
| Antibody . | Fluorescence Intensity (arbitrary units) . | |
|---|---|---|
| . | Nonmigrating Cells . | Transmigrated Cells . |
| IgG control (FITC) | 0.18 ± 0.01 | 0.20 ± 0.02 |
| IgG control (PE) | 0.21 ± 0.01 | 0.23 ± 0.01 |
| CD34 (PE) | 12.7 ± 3.8 | 14.1 ± 5.0 |
| CD38 (FITC) | 6.6 ± 0.8 | 10.0 ± 1.0* |
| L-selectin (FITC) | 4.1 ± 0.7 | 0.8 ± 0.1* |
| VLA-4 (FITC) | 2.3 ± 0.3 | 2.9 ± 0.5 |
| LFA-1 (PE) | 1.0 ± 0.4 | 1.1 ± 0.3 |
A total of 1 × 106 CD34+ cells were added to the upper chamber of the transmigration system. Nonmigrating and transmigrated cells were recovered after 24 hours and analyzed by flow cytometry.
P < .05.