Effects of IL-4 and IL-13 on Immature NK Cell Colonies
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7, IL-15, SF | 1 ± 1 | 1 ± 1 | 24 ± 2 (97)4-150 |
| IL-4 (10 ng/mL), IL-7, IL-15, SF | 1 ± 1 | 2 ± 1 | 0 (0) | ||
| IL-13 (10 ng/mL), IL-7, IL-15, SF | 1 ± 1 | 2 ± 1 | 22 ± 1 (86) | ||
| IL-13 (50 ng/mL), IL-7, IL-15, SF | 0 ± 1 | 1 ± 1 | 16 ± 2 (65) | ||
| CD25+c-kit+ | 500 | IL-7, IL-15, SF | 0 | 0 | 27 ± 1 (107) |
| IL-4 (10 ng/mL), IL-7, IL-15, SF | 0 ± 1 | 1 ± 1 | 0 (0) | ||
| IL-13 (10 ng/mL), IL-7, IL-15, SF | 0 | 1 ± 1 | 26 ± 1 (104) | ||
| IL-13 (50 ng/mL), IL-7, IL-15, SF | 0 | 0 | 18 ± 2 (80) | ||
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7, IL-15, SF | 1 ± 1 | 1 ± 1 | 24 ± 2 (97)4-150 |
| IL-4 (10 ng/mL), IL-7, IL-15, SF | 1 ± 1 | 2 ± 1 | 0 (0) | ||
| IL-13 (10 ng/mL), IL-7, IL-15, SF | 1 ± 1 | 2 ± 1 | 22 ± 1 (86) | ||
| IL-13 (50 ng/mL), IL-7, IL-15, SF | 0 ± 1 | 1 ± 1 | 16 ± 2 (65) | ||
| CD25+c-kit+ | 500 | IL-7, IL-15, SF | 0 | 0 | 27 ± 1 (107) |
| IL-4 (10 ng/mL), IL-7, IL-15, SF | 0 ± 1 | 1 ± 1 | 0 (0) | ||
| IL-13 (10 ng/mL), IL-7, IL-15, SF | 0 | 1 ± 1 | 26 ± 1 (104) | ||
| IL-13 (50 ng/mL), IL-7, IL-15, SF | 0 | 0 | 18 ± 2 (80) | ||
CD25−c-kit+ and CD25+c-kit+ cells sorted from day 14 fetal thymocytes were cultured in the presence of cytokines. Colonies were scored on day 14 of culture. Data represent mean ± SD of quadruplicate cultures.
Total number of colonies in 4 plates is shown in parentheses.