Effects of IL-2 and IL-15 on the Formation of Immature NK Cell Colonies
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7, SF | 0 | 1 ± 1 | 11 ± 1 (42)3-150 |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 19 ± 1 (76) | ||
| IL-15, IL-7, SF | 0 | 0 | 18 ± 2 (71) | ||
| IL-2, IL-15, IL-7, SF | 0 | 1 ± 1 | 19 ± 2 (75) | ||
| CD25+c-kit+ | 500 | IL-7, SF | 0 | 1 ± 1 | 12 ± 1 (49) |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 24 ± 1 (94) | ||
| IL-15, IL-7, SF | 0 | 1 ± 1 | 19 ± 2 (76) | ||
| IL-2, IL-15, IL-7, SF | 0 | 1 ± 1 | 24 ± 1 (95) | ||
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7, SF | 0 | 1 ± 1 | 11 ± 1 (42)3-150 |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 19 ± 1 (76) | ||
| IL-15, IL-7, SF | 0 | 0 | 18 ± 2 (71) | ||
| IL-2, IL-15, IL-7, SF | 0 | 1 ± 1 | 19 ± 2 (75) | ||
| CD25+c-kit+ | 500 | IL-7, SF | 0 | 1 ± 1 | 12 ± 1 (49) |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 24 ± 1 (94) | ||
| IL-15, IL-7, SF | 0 | 1 ± 1 | 19 ± 2 (76) | ||
| IL-2, IL-15, IL-7, SF | 0 | 1 ± 1 | 24 ± 1 (95) | ||
CD25−c-kit+ or CD25+c-kit+ cells from day 14 fetal thymocytes were cultured in the presence of designated cytokines. Colonies were scored on day 14 of culture. Data represent mean ± SD of quadruplicate cultures.
Total number of immature NK cell colonies in 4 plates is shown in parentheses.