Minimal Cytokine Requirement of Immature NK Colonies
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7 | 0 | 0 | 0 (0)* |
| IL-15 | 0 | 0 | 0 (0) | ||
| SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15 | 0 | 0 | 0 (0) | ||
| IL-7, SF | 0 ± 1 | 1 ± 1 | 11 ± 2 (45) | ||
| IL-15, SF | 0 | 1 ± 1 | 0 (0) | ||
| IL-7, IL-15, SF | 1 ± 1 | 0 ± 1 | 20 ± 2 (79) | ||
| CD25+c-kit+ | 500 | IL-7 | 0 | 0 | 0 (0) |
| IL-15 | 0 | 0 | 0 (0) | ||
| SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15 | 0 | 0 | 0 (0) | ||
| IL-7, SF | 0 ± 1 | 0 ± 1 | 12 ± 3 (46) | ||
| IL-15, SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15, SF | 1 ± 1 | 1 ± 1 | 16 ± 1 (64) | ||
| Cells Plated . | Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|---|
| Type . | No./Dish . | . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | IL-7 | 0 | 0 | 0 (0)* |
| IL-15 | 0 | 0 | 0 (0) | ||
| SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15 | 0 | 0 | 0 (0) | ||
| IL-7, SF | 0 ± 1 | 1 ± 1 | 11 ± 2 (45) | ||
| IL-15, SF | 0 | 1 ± 1 | 0 (0) | ||
| IL-7, IL-15, SF | 1 ± 1 | 0 ± 1 | 20 ± 2 (79) | ||
| CD25+c-kit+ | 500 | IL-7 | 0 | 0 | 0 (0) |
| IL-15 | 0 | 0 | 0 (0) | ||
| SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15 | 0 | 0 | 0 (0) | ||
| IL-7, SF | 0 ± 1 | 0 ± 1 | 12 ± 3 (46) | ||
| IL-15, SF | 0 | 0 | 0 (0) | ||
| IL-7, IL-15, SF | 1 ± 1 | 1 ± 1 | 16 ± 1 (64) | ||
CD25−c-kit+ or CD25+c-kit+ cells were prepared by FACS sorting from day 14 fetal thymocytes. Culture was performed in the presence of designated cytokines. Colonies were scored on day 14 of culture. Data represent mean ± SD of quadruplicate culture.
Total number of immature NK cell colonies in 4 dishes is shown in parentheses.