Colony Formation From Subpopulation of Fetal Thymocytes Separated on the Basis of CD25/c-kit Expression
| Cells Plated . | No. of Colonies . | |||
|---|---|---|---|---|
| Type . | No./Dish . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | 1 ± 1 | 1 ± 1 | 20 ± 2 (79)* |
| CD25+c-kit+ | 500 | 0 ± 1 | 1 ± 1 | 19 ± 1 (75) |
| CD25−c-kit− | 1,500 | 0 | 1 ± 1 | 2 ± 1 (8) |
| CD25+c-kit− | 4,000 | 0 | 0 ± 1 | 3 ± 1 (11) |
| Cells Plated . | No. of Colonies . | |||
|---|---|---|---|---|
| Type . | No./Dish . | GM . | Mast . | NK . |
| CD25−c-kit+ | 300 | 1 ± 1 | 1 ± 1 | 20 ± 2 (79)* |
| CD25+c-kit+ | 500 | 0 ± 1 | 1 ± 1 | 19 ± 1 (75) |
| CD25−c-kit− | 1,500 | 0 | 1 ± 1 | 2 ± 1 (8) |
| CD25+c-kit− | 4,000 | 0 | 0 ± 1 | 3 ± 1 (11) |
Designated populations of cells were prepared by FACS sorting from day 14 fetal thymocytes of BDF1 mice and cultured with IL-7, IL-15, and SF in methylcellulose. Colonies were counted on day 14 of culture. Data represent mean ± SD of quadruplicate cultures.
Total number of immature NK cell colonies in four plates is shown in parentheses.